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Key Points Question What advice regarding the workplace and management strategies are perceived as useful to women leaders in academic medicine? Findings In this qualitative study based on responses from 40 women leaders at the Mayo Clinic, we found that advice could be grouped into 4 categories: leadership styles are perceived as having gendered qualities, a strategic process is required to gain leadership skills, conflicts between personal life and the workplace will occur and should not be a deterrent, and leadership pathways for women involve inevitable hurdles. Meaning The findings of this qualitative study suggest that a long-term view of attaining gender equity for women leaders at academic medical centers will require a combination of programs, including disruptive institutional initiatives and grassroots efforts; 1 approach involves highlighting and disseminating career advice received by female leaders, particularly advice that others have found helpful.