SVMMARYGrowth, COj assimilation rate {A), leaf conductance {g), transpiration efficiency (W = ratio biomass production/plant water use) and carbon isotope discrimination (A) were assessed in maritime pine (Pinus pinaster Ait.) and pedunculate oak (Quercus robur L.) grown on a sand-peat mixture with three levels of fertilization : FlOO, optimal complete fertilization; F25, 25 "o of the optima! fertilizer supply; FO, no fertilization. Leaf phosphorus (P) and potassium (K) concentrations were affected little by the diminishing nutrient availability. Reduced fertilization decreased plant nitrogen (N) concentration in both species but leaf N concentration was less affected in oak than in pine. In pine W was markedly reduced in response to reduced leaf or whole plant N concentration, which was consistent with the sharp decrease also observed for plant intrinsic water-use efficiency (ratio A/g) both at the instantaneous (gas exchange data) and time-integrated (A/g derived from A measurements) levels. In this species, lowered W in the N deficient conditions was primarily associated with enhanced values of g. The existence of such a stomatal response pattern, confirmed by the increase in plant transpiration between FlOO and F25, has not been reported before. In oak, both A and g were decreased in F25 and FO as compared with FlOO. W was not affected -and instantaneous as well as time-integrated A/g values were oni}' slightly decreased -in relation to decreasing plant N concentration. For FlOO, no difference in W was noticed between pine and oak though the A values were 2-6°^ lower in oak. We speculate that this discrepancy was linked with higher plant-carbon losses through processes like respiration, fine-root mortality or root exudation in oak. The isotopic approach proved useful for assessing the effects of nutritional status on W^ but has to be used with caution w hen comparing different species.Key words: Pinuspinaster (maritime pine), Quercus robur [pedunculate o-dk), nitrogen deficiency, gas exchange and water-use efficiency, carbon isotope discrimination.
Leaf conductance, water relations, growth, and abscisic acid (ABA) concentrations in xylem sap, root apices and leaves were assessed in oak seedlings (Quercus robur L.) grown with a root system divided between two compartments and subjected to one of four treatments: (a) well watered, WW; (b) half of root system exposed to soil drying and half kept well watered, WD; (c) whole root system exposed to drought, DD; and (d) half of root system severed, RE. Sharp decreases in plant stomatal conductance, leaf water potential, hydraulic conductance and leaf growth were observed during DD treatment. No significant differences in plant leaf water potential and stomatal conductance were detected between the WW and WD treatments. Nevertheless, the WD treatment resulted in inhibition of leaf expansion and stimulation of root elongation only in the well-watered compartment. Abscisic acid concentrations did not change in leaves, root tips, or xylem sap of WD- compared to WW-treated plants. Increased concentrations of ABA were observed in xylem sap from DD-treated plant roots, but the total flux of ABA to shoots was reduced compared to that in WW-treated plants, because of decreases in transpiration flux. Similar plant responses to the WD and RE treatments indicate that the responses observed in the WD-treated plants were probably not triggered by a positive signal originating from drying roots.
Seedlings of Betula pendula Roth were grown with their root systems separated between two soil compartments. Four treatments were imposed: (i) adequate irrigation in both compartments (WW, controls); (ii) adequate irrigation in one compartment and drought in the other compartment (WD); (iii) drought in both compartments (DD); and (iv) half of the root system severed and the remainder kept well-watered (root excision, RE). Predawn leaf water potential, stomatal conductance, soil-to-leaf specific hydraulic conductance, and root and leaf growth decreased in DD-treated seedlings, which also displayed severe leaf shedding (30% loss in leaf area). The DD treatment also resulted in increased concentrations of abscisic acid (ABA) and its glucose ester in the xylem sap of roots and shoots compared to concentrations in control seedlings (about 200 versus 20 nM). Despite the difference in xylem sap concentrations, total ABA flux to the shoots was similar in the two treatments (1-2 pmol ABA m(-2) leaf area s(-1)) as a result of reduced transpiration in the DD-treated seedlings. Compared with root growth in control plants, root growth increased in the RE-treated plants and decreased in the drying compartment of the WD treatment; however, the RE and WD treatments only slightly reduced leaf expansion, and had no detectable effects on shoot water relations or ABA concentrations of the root and shoot xylem sap. We conclude that short-term soil water depletion affecting only 50% of the root system does not cause a measurable stress response in birch shoots, despite root growth cessation in the fraction of drying soil.
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