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Purpose To investigate fluorescence lifetime imaging ophthalmoscopy (FLIO) in neovascular AMD and pigment epithelial detachments (PEDs). Methods A total of 46 eyes with PEDs (>350 μm) as well as age-matched healthy controls were included in this study. We found 28 eyes showed neovascular AMD (nvAMD), and 17 had nonneovascular (dry) AMD (dAMD). The Heidelberg Engineering FLIO excited fluorescence at 473 nm. Fluorescence decays were detected in two spectral channels (498–560 nm; 560–720 nm) to determine fluorescence lifetimes of endogenous fluorophores in their specific spectral emission ranges. Mean fluorescence lifetimes (τ m ) were investigated. Multimodal imaging was reviewed by two ophthalmologists who circumscribed and classified PEDs as either serous ( n = 4), hemorrhagic ( n = 4), fibrovascular ( n = 16), drusenoid ( n = 17), or mixed ( n = 5). Blood samples from a healthy subject and a patient with PED were investigated in a quartz cuvette. Results Eyes with nvAMD show similar FLIO patterns to dAMD: ring-shaped prolongations of τ m 3 to 6 mm from the fovea. Different PED-forms show characteristic τ m , while serous and hemorrhagic PEDs exhibit shortened τ m , drusenoid PEDs show prolonged τ m , and τ m in fibrovascular PEDs is variable. Areas corresponding to sub-/intraretinal fluid display shortened τ m . Ex vivo studies of blood also show short τ m . Conclusions The previously described dAMD-related FLIO pattern is also present in nvAMD. Short τ m in serous, fibrovascular, and hemorrhagic PEDs as well as sub/intraretinal fluid may disrupt this pattern. FLIO appears to differentiate between PEDs, hemorrhage, and fluid. Additionally, ex vivo studies of human blood help to better interpret FLIO images.

The treatment of patients with osteoporosis and spinal abnormalities that require surgical intervention is difficult because of the challenge of achieving fixation in osteoporotic bone. As the population ages, this challenge is becoming a common problem in the field of spinal surgery. Although numerous publications exist about the biomechanical benefits of various fixation devices and techniques, no standard of care has emerged that offers a clear method for accomplishing spinal stabilization in such patients. This case presents the failure mode of cement-augmented pedicle screws in a patient with severe osteoporosis, a description of the methods used to attain fixation and spinal stability during the revision surgery, and the outcome achieved for the patient 1 year after surgery. An 82-year-old female with a T9 burst fracture and a history of osteoporosis underwent minimally invasive instrumentation from T5 to T12, fusion from T7 to T11, and decompression from T8 to T10. Four weeks after surgery, the patient returned to the hospital because of back pain. Imaging studies showed that the pedicle screws at T11 and T12, which were augmented with polymethylmethacrylate (PMMA), had pulled out of the vertebral bodies. The pedicle screws failed by disengaging from the PMMA and displacing posteriorly and inferiorly. The PMMA did not appear to move during this process. A revision surgery was performed, in which the posterior construct was extended caudally and cephalad, the pedicle screws were augmented with PMMA, and a titanium hook and woven polyester band were used to increase the points of fixation. At 1-year follow-up after revision, our patient showed radiographic evidence of fusion, and the construct continued to maintain stability in the osteoporotic spine.