Abstract. The recurrence of the same types of sequence stratigraphic surface through geologic time defines cycles of change in accommodation or sediment supply, which correspond to sequences in the rock record. These cycles may be symmetrical or asymmetrical, and may or may not include all types of systems tracts that may be expected within a fully developed sequence. Depending on the scale of observation, sequences and their bounding surfaces may be ascribed to different hierarchical orders. Stratal stacking patterns combine to define trends in geometric character that include upstepping, forestepping, backstepping and downstepping, expressing three types of shoreline shift: forced regression (forestepping and downstepping at the shoreline), normal regression (forestepping and upstepping at the shoreline) and transgression (backstepping at the shoreline). Stacking patterns that are independent of shoreline trajectories may also be defined on the basis of changes in depositional style that can be correlated regionally. All stratal stacking patterns reflect the interplay of the same two fundamental variables, namely accommodation (the space available for potential sediment accumulation) and sediment supply. Deposits defined by specific stratal stacking patterns form the basic constituents of any sequence stratigraphic unit, from sequence to systems tract and parasequence. Changes in stratal stacking patterns define the position and timing of key sequence stratigraphic surfaces. Precisely which surfaces are selected as sequence boundaries varies as a function of which surfaces are best expressed within the context of the depositional setting and the preservation of facies relationships and stratal stacking patterns in that succession. The high degree of variability in the expression of sequence stratigraphic units and bounding surfaces in the rock record means ideally that the methodology used to analyze their depositional setting should be flexible from one sequence stratigraphic approach to another. Construction of this framework ensures the success of the method in terms of its objectives to provide a process-based understanding of the stratigraphic architecture. The purpose of this paper is to emphasize a standard but flexible methodology that remains objective.

An understanding of the structural complexity of mineralised tissues is fundamental for exploration into the field of diagenesis. Here we review aspects of current and past research on bone and tooth diagenesis using the most comprehensive collection of literature on diagenesis to date. Environmental factors such as soil pH, soil hydrology and ambient temperature, which influence the preservation of skeletal tissues are assessed, while the different diagenetic pathways such as microbial degradation, loss of organics, mineral changes, and DNA degradation are surveyed. Fluctuating water levels in and around the bone are the most harmful for preservation and lead to rapid skeletal destruction. Diagenetic mechanisms are found to work in conjunction with each other, altering the biogenic composition of skeletal material. This illustrates that researchers must examine multiple diagenetic pathways to fully understand the post-mortem interactions of archaeological skeletal material and the burial environment.

Little is known regarding the first people to enter the Americas and their genetic legacy. Genomic analysis of the oldest human remains from the Americas showed a direct relationship between a Clovis-related ancestral population and all modern Central and South Americans as well as a deep split separating them from North Americans in Canada. We present 91 ancient human genomes from California and Southwestern Ontario and demonstrate the existence of two distinct ancestries in North America, which possibly split south of the ice sheets. A contribution from both of these ancestral populations is found in all modern Central and South Americans. The proportions of these two ancestries in ancient and modern populations are consistent with a coastal dispersal and multiple admixture events.