Scaffolds for heart valve tissue engineering must function immediately after implantation but also need to tolerate cell infiltration and gradual remodeling. We hypothesized that moderately cross-linked collagen scaffolds would fulfill these requirements. To test our hypothesis, scaffolds prepared from decellularized porcine pericardium were treated with penta-galloyl glucose (PGG), a collagen-binding polyphenol, and tested for biodegradation, biaxial mechanical properties, and in vivo biocompatibility. For controls, we used un-cross-linked scaffolds and glutaraldehyde-treated scaffolds. Results confirmed complete pericardium decellularization and the ability of scaffolds to encourage fibroblast chemotaxis and to aid in creation of anatomically correct valve-shaped constructs. Glutaraldehyde cross-linking fully stabilized collagen but did not allow for tissue remodeling and calcified when implanted subdermally in rats. PGG-treated collagen was initially resistant to collagenase and then degraded gradually, indicating partial stabilization. Moreover, PGG-treated pericardium exhibited excellent biaxial mechanical properties, did not calcify in vivo, and supported infiltration by host fibroblasts and subsequent matrix remodeling. In conclusion, PGG-treated acellular pericardium is a promising scaffold for heart valve tissue engineering.

This article presents an analytical and experimental investigation of energy harvesting via parametrically excited cantilever beams. To that end, we consider a lumped-parameter non-linear model that describes the first-mode dynamics of a parametrically excited cantilever-type harvester. The model accounts for the beam's geometric and inertia non-linearities as well as non-linearities representing air drag. Using the method of multiple scales, we obtain approximate analytical expressions describing the beam response, voltage drop across a purely resistive load, and output power in the vicinity of the first principle parametric resonance. Using these expressions, we study the effect of the electromechanical coupling and load resistance on the output power. We show that these parameters play an imperative role in determining the magnitude of the output power and characterizing the broad-band properties of the harvester. Specifically, we show that the region of parametric instability wherein energy can be harvested shrinks as the coupling coefficient increases. Furthermore, we show that there exists a coupling coefficient beyond which the peak power decreases. We also demonstrate that there is a critical excitation level below which no energy can be harvested. The amplitude of this critical excitation increases with the coupling coefficient and is maximized for a given load resistance. Theoretical findings that were compared to experimental results show good agreement and reflect the general trends.

Tissue-engineered vascular grafts require elastic, acellular porous scaffolds with controlled biodegradability and properties matching those of natural arteries. Elastin would be a desirable component for such applications, but elastin does not easily regenerate experimentally. Our approach is to develop tubular elastin scaffolds using decellularization and removal of collagen from porcine carotid arteries (*5 mm diameter) using alkaline extraction. Because elastin is susceptible to rapid degeneration after implantation, scaffolds were further treated with penta-galloyl glucose (PGG), an established polyphenolic elastin-stabilizing agent. Scaffolds were compared in vitro with detergent-decellularized arteries for structure, composition, resistance to degradation, mechanical properties, and cytotoxicity and in vivo for cell infiltration and remodeling potential. Results showed effective decellularization and almost complete collagen removal by alkaline extraction. PGG-treated elastin scaffolds proved to be resistant to elastase digestion in vitro, maintained their cylindrical shapes, showed high resistance to burst pressures, and supported growth of endothelial cells and fibroblasts. In vivo results showed that PGG treatment reduced the rate of elastin biodegradation and controlled cell infiltration but did not hamper new collagen and proteoglycan deposition and secretion of matrix-degrading proteases. Alkali-purified, PGG-treated tubular arterial elastin scaffolds exhibit many desirable properties to be recommended for clinical applications as vascular grafts.

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In this work, inspired by music playing harmonicas, we conduct a conceptual investigation of a coupled aero-electromechanical system for wind energy harvesting. The system consists of a piezoelectric cantilever unimorph structure embedded within an air chamber to mimic the vibration of the reeds in a harmonica when subjected to air flow. In principle, when wind blows into the air chamber, the air pressure in the chamber increases and bends the cantilever beam opening an air path between the chamber and the environment. When the volumetric flow rate of air past the cantilever is large enough, the energy pumped into the structure via the nonlinear pressure forces offset the intrinsic damping in the system setting the beam into self-sustained limit-cycle oscillations. These oscillations induce a periodic strain in the piezoelectric layer which produces a voltage difference that can be channeled into an electric load. Unlike traditional vibratory energy harvesters where the excitation frequency needs to match the resonant frequency of the device for efficient energy extraction, the nonlinearly coupled aero-elasto dynamics of this device guarantees autonomous vibration of the cantilever beam near its natural frequency as long as the volumetric flow rate is larger than a certain threshold. Experimental results are presented to demonstrate the ability of this device to harvest wind energy under normal wind conditions.