Sepsis is a life-threatening condition and often associated with multiple organ failure. Nuclear-enriched abundant transcript 1 (NEAT1), a member of the long non-coding RNAs (lncRNAs), was reported to be involved in the regulation of sepsis progression. However, its precise regulatory mechanism needs to be further explored. In this study, the cell-counting kit-8 assay was used to check cell viability. The quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression levels of NEAT1, miR-370-3p and Interleukin 1 receptor associated kinase 2 (Irak2). Flow cytometry assay and ELISA were used to check cell apoptosis and the concentrations of inflammatory cytokines, respectively. The starBase was used to predict binding sites between miR-370-3p and NEAT1 or Irak2 and the dual-luciferase reporter assay was performed to verify the interaction. The protein level of Irak2 in samples was measured by western blot. The high concentration of lipopolysaccharide (LPS) led to the high death ratio of RAW 264.7 and HL-1 cells. NEAT1 and Irak2 were upregulated in sepsis tissues and LPS-induced RAW 264.7 and HL-1 cells, opposite to the expression of miR-370-3p. In addition, knockdown of NEAT1 promoted viability, suppressed apoptosis and reduced the expression of inflammatory cytokines in LPS-induced RAW 264.7 and HL-1 cells. Moreover, we found that miR-370-3p interacted with NEAT1 and targeted the 3′UTR of Irak2. Further research indicated that downregulation of miR-370-3p or upregulation of Irak2 rescued NEAT1 silencing-mediated inhibitory effect on sepsis progression. Knockdown of NEAT1 hampered sepsis progression by downregulating Irak2 via interacting with miR-370-3p in LPS-induced RAW 264.7 and HL-1 cells.

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Context Kang-ai injection (KAI) is an authorized herbal medicine used in cancer treatment. However, its clinical efficacy in hepatocellular carcinoma (HCC) has not been investigated thoroughly. Objective To systematically evaluate the efficacy and safety of KAI in patients with HCC. Materials and methods The Web of Science, PubMed, Cochrane Library, EMBASE, CBM, CNKI, VIP and Wanfang databases were systematically searched (date range: inception to December 2020) using the key terms ‘Kang-ai injection’ and ‘hepatocellular carcinoma’. The current analysis included controlled clinical trials that compared the efficacy and safety of the combination of KAI and conventional treatment (CT) with CT alone for HCC. The current study estimated the pooled risk ratio (RR) with 95% confidence intervals (CI). Results Data pertaining to 35 trials with 2501 HCC patients were analysed. The results revealed that the combination of KAI and CT was associated with significantly superior objective response rate (RR = 1.57, 95% CI = 1.43–1.73), disease control rate (RR = 1.18, 95% CI = 1.10–1.26), and quality of life (RR = 2.40, 95% CI = 1.79–3.23), compared to CT alone. The administration of KAI significantly alleviated most of the adverse effects caused by CT, including nausea and vomiting, liver damage, peripheral neurotoxicity, fever, abdominal pain, alopecia, increased bilirubin levels, leukopoenia, and reduction in haemoglobin levels ( p < 0.05, for all). Conclusions The current meta-analysis indicates that a combination of CT and KAI could be more effective in improving the clinical efficacy of the treatment of HCC, compared to CT alone.

Review question / Objective: Review q u e s t i o n : I s S M L H I e ff e c t i v e o n neurological deficit score (NIHSS), activity of daily living (ADL), hemorheology and blood lipid indexes in patients with acute ischemic stroke (AIS)? Objective: A systematic review and meta-analysis will be performed to systematically evaluate the efficacy of SMLHI on NIHSS, ADL, INPLASY1 International Platform of Registered Systematic Review and Meta-analysis Protocols INPLASY PROTOCOL Efficacy of Salviae miltiorrhizae and ligustrazine hydrochloride injection on NIHSS, activity of daily living, hemorheology and blood lipid indexes in patients with acute ischemic stroke: a protocol for systematic review and meta analysis Sun, CH 1 ; Wan, HH 2 ; Yang, J 3 ; Zhou, CH 4 ; Wang, XL 5 . To cite: Sun et al. Efficacy of Salviae miltiorrhizae and ligustrazine hydrochloride injection on NIHSS, activity of daily living, hemorheology and blood lipid indexes in patients with acute ischemic stroke: a protocol for systematic review and meta analysis. Inplasy protocol 202180039.