Few studies explore the effects of nitrogen forms on exotic plant invasions, and all of them are conducted from the perspective of nitrogen form utilization without considering the effects of ammonium toxicity. The invasive plant Xanthium strumarium prefers to use nitrate, while its native congener X. sibiricum prefers to use ammonium, and the invader is more sensitive to high ammonium based on our preliminary observations. To further reveal the effects of nitrogen forms on invasiveness of X. strumarium, we studied the difference and related physiological mechanisms in sensitivity to ammonium between these species. With increasing ammonium, total biomass, root to shoot ratio and leaf chlorophyll content of X. strumarium decreased, showing ammonium toxicity. For X. sibiricum, however, ammonium toxicity did not occurr. With increasing ammonium, ammonium concentration increased in leaves and roots of X. strumarium, which is associated with the decreased activities of glutamine synthetase and glutamate synthase and the increased ammonium uptake; and consequently the contents of hydrogen peroxide and malondialdehyde also increased, which is associated with the decreased contents of reduced glutathione and ascorbic acid. By contrast, the abilities of ammonium assimilation and antioxidation of X. sibiricum were less affected by the increase of ammonium, and the contents of ammonium nitrogen, hydrogen peroxide and malondialdehyde in leaves and roots were significantly lower than those in X. strumarium. Our results indicate that ammonium accumulation and oxidative damage may be the physiological mechanisms for the ammonium toxicity of X. strumarium, providing a possible explanation that it generally invades nitrate-dominated and disturbed habitats and a theoretical basis for future studies on the control of invasive plants by regulating soil nitrogen.