No abstract

In the past few years, some studies claimed that annihilating dark matter with mass ∼ 10−100 GeV can explain the GeV gamma-ray excess in our Galaxy. However, recent analyses of the Fermi-LAT and radio observational data rule out the possibility of the thermal relic annihilating dark matter with mass m ≤ 100 GeV for some popular annihilation channels. By using the new observed radio data of the Andromeda galaxy, we rule out the existence of ∼ 100 − 300 GeV thermal relic annihilating dark matter for ten annihilation channels. The lower limits of annihilating dark matter mass are improved to larger than 330 GeV for the most conservative case, which is a few times larger than the current best constraints. Moreover, these limits strongly disfavor the benchmark model of weakly interacting massive particle (WIMP) produced through the thermal freeze-out mechanism.

In hepatocellular carcinoma (HCC) patients with extrahepatic metastasis, the lung is the most frequent site of metastasis. However, how the lung microenvironment favors disseminated cells remains unclear. Here, it is found that nidogen 1 (NID1) in metastatic HCC cell‐derived extracellular vesicles (EVs) promotes pre‐metastatic niche formation in the lung by enhancing angiogenesis and pulmonary endothelial permeability to facilitate colonization of tumor cells and extrahepatic metastasis. EV‐NID1 also activates fibroblasts, which secrete tumor necrosis factor receptor 1 (TNFR1), facilitate lung colonization of tumor cells, and augment HCC cell growth and motility. Administration of anti‐TNFR1 antibody effectively diminishes lung metastasis induced by the metastatic HCC cell‐derived EVs in mice. In the clinical perspective, analysis of serum EV‐NID1 and TNFR1 in HCC patients reveals their positive correlation and association with tumor stages suggesting the potential of these molecules as noninvasive biomarkers for the early detection of HCC. In conclusion, these results demonstrate the interplay of HCC EVs and activated fibroblasts in pre‐metastatic niche formation and how blockage of their functions inhibits distant metastasis to the lungs. This study offers promise for the new direction of HCC treatment by targeting oncogenic EV components and their mediated pathways.

Acupressure seems to be effective in providing immediate relief of pretreatment anxiety among adults, and has a medium effect size. However, conflicting results were found for the improvements on physiological indicators. More rigorous reporting, including allocation concealment procedure, is needed to strengthen the results.