It is known that serial correlation within time series at sites and cross-correlation among sites in a specific region will influence the ability of statistical tests to assess the field significance of trends over the region. However, serial and/or cross-correlation has been ignored in field trend-analyses. This study attempts to develop a methodology that takes into account both serial and cross-correlation in the assessment of the field significance of trends. The regional average Mann-Kendall (RAMK) statistic is used to represent the regional properties of trends at a regional scale. The null distribution of the RAMK statistic is derived on the basis that the joint probability distribution of m independent normal variables is also normally distributed. The variance of the RAMK statistic is then modified by serial and cross-correlation. The applicability of the method was demonstrated by applying it to assess the field significance of trends in annual mean, annual maximum, and annual minimum daily streamflow from 1967 to 1996 in ten major homogeneous climate regions of Canada. The results indicate that the method developed provides more accurate assessment of the field significance of trends than that without consideration of serial and cross-correlation.At the significance level of 0.10, annual mean daily flow increased significantly in the region of Yukon and northern BC mountains whereas it decreased significantly in the Pacific and the Prairie regions. Annual maximum daily flow decreased significantly across southern Canada, except in the Pacific region. Annual minimum daily flow decreased significantly in the Pacific region and in southeastern Canada, with the exception of the region of Great Lakes and St Lawrence river basin, whereas it increased significantly in the region of Yukon and northern BC mountains.

The temporal distribution patterns of bacterial communities, as an important group in mountain soil, are affected by various environmental factors. To improve knowledge regarding the successional seasonal dynamics of the mountain soil bacterial communities, the rhizospheric soil of a 30-year-old natural secondary Pinus tabulaeformis forest, located in the high-altitude (1900 m a.s.l.) of the temperate Qinling Mountains, was sampled and studied during four different seasons. The bacterial community composition and structure in the rhizospheric soil were studied using an Illumina MiSeq Sequencing platform. Furthermore, the edaphic properties and soil enzymatic activities (urease, phosphatase, and catalase) were measured in order to identify the main impact factors on the soil bacterial community. According to the results, all of the edaphic properties and soil enzymatic activities were significantly affected by the seasonal changes, except for the C/N ratio. Although the biomasses of soil bacterial communities increased during the summer and autumn (warm seasons), their Shannon diversity and Pielou’s evenness were decreased. Proteobacteria, Acidobacteria, Actinobacteria, Planctomycetes, and Bacteroidetes were the predominant bacterial groups in all of the soil samples, and the genera of Ktedonobacter, Sphingobium as well as an unclassified member of the Ktedonobacteria were the keystone taxa. The composition and structure of soil bacterial communities were strongly impacted by the edaphic properties, especially the temperature, moisture, ammoniacal nitrogen, available phosphorus and total phosphorus which were the crucial factors to drive the temporal distribution of the soil bacterial community and diversity. In conclusion, the soil temperature, moisture and the nutrients N and P were the crucial edaphic factors for shaping the rhizospheric soil bacterial communities as season and climate change in a P. tabulaeformis forest of Qinling Mountains.