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The crack presence causes nonlinear stress distributions along the sections of a beam, which change the neutral axis of the sections and further affect the beam stiffness. Thus, this paper presents a method for the stiffness estimation of cracked beams based on the stress distributions. First, regions whose stresses are affected by the crack are analyzed, and according to the distance to the crack, different nonlinear stress distributions are modeled for the effect regions. The inertia moments of section are evaluated by substituting these stress distributions into the internal force equilibrium of section. Then the finite-element technique is adopted to estimate the stiffness of the cracked beam. The estimated stiffness is used to predict the displacements of simply supported beams with a crack, and the results show that both static and vibrational displacements are accurately predicted, which indicates that the estimated stiffness is precise enough. Besides, as the section shape of beam is not limited in the process of modeling the stress distributions, the method could be applicable not only to the stiffness estimation of cracked beams with a rectangular section, but also to that of the beams with a T-shaped section if the crack depth ratio is not larger than 0.7.

In recent years, porcine reproductive and respiratory syndrome virus (PRRSV) strains have been experiencing extensive recombination in Chinese swine farms. This recombination usually happens in NADC30/34 strains and highly pathogenic (HP) PRRSV strains. This study identified a new PRRSV isolate that shared 99% and 99.1% nucleotide identity with CH-1a and CH-1R at the genomic level, respectively. After purification by viral plaque assay, this isolate was named PRRSV CSR1801. The isolate did not experience any recombination with other PRRSV strains common in swine herd epidemics in China, which means it still maintains the stable features of the classical PRRSV strain and did not easily recombine with other PRRSV strains. Further analysis of the pathogenicity of the PRRSV isolate CSR1801 was performed in piglets. The results indicated that none of the inoculated piglets showed the typical clinical manifestations of PRRS, which presented with runny noses, rough back hair, rectal temperatures always below 40.5 °C, and no deaths. Additionally, no obvious histopathological lesions such as severe interstitial pneumonia could be observed in the lungs of the piglets. Hence, the PRRSV isolate CSR1801 should be classified as a classical-like PRRSV strain. This classical PRRSV strain showed genetic stability and maintained low pathogenicity. This study may provide new clues for further understanding the genetic evolution and pathogenicity of PRRSV and may also be an important reference for the prevention and control of PRRS in swine farms.

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