Background: The skin is the first line of defense against microbial and chemical agents. Keratinocytes, represent the major component of the skin. Storax is thought to have antioxidant, anti-inflammatory and antimicrobial effects. some substances in storax have a cytotoxic effect and that storax can be a source of oxidative stress. Boron compounds have a wide physiological effect on biological systems at low concentrations, are toxic at high concentrations. The aim of this study is to evaluate the oxidative effect of storax on boron compounds treated HaCaT keratinocytes in vitro. Methods: In order to determine the effect of boron compounds on cell viability and 50% lethal dose, the MTT method was employed, and the IC50 dose was found to be 1000 μg/ml borax and 250 μg/ml colemanite at 24th hour. To determine the antioxidant activity of storax cells treated with borax and colemanite with or without storax and then the oxidative stress index, SOD, GPx and MDA levels were evaluated with ELISA. Results: storax reduces the oxidative stress index through GPX, SOD and MDA activities. Conclusion: When all the results are evaluated, the idea arises, that storax can be used as a possible therapeutic agent in skin.