Lagoas temporárias são ambientes efêmeros pouco estudados. O objetivo deste trabalho foi inventariar a comunidade de amebas testáceas encontradas em dois biótopos (plâncton e perifíton) de um corpo aquático temporário contaminado por dejetos orgânicos. As coletas foram realizadas entre junho e setembro de 2018 na Fazenda Periperi, Vitória da Conquista, Bahia. Foram coletadas 51 amostras planctônicas e 12 perifíticas. As análises físico-químicas foram realizadas in situ e as bacteriológicas em laboratório através do kit Colilert®. A água deste ambiente é alcalina, com elevados valores de temperatura, sólidos totais dissolvidos e condutividade elétrica. Apresentou baixos teores de oxigênio dissolvido e transparência, refletindo a elevada biomassa fitoplanctônica presente. Os valores de coliformes totais e termotolerantes demonstraram contaminação fecal na água. Foram registrados 32 taxa de amebas testáceas distribuídos em 11 gêneros e oito famílias. A maior riqueza foi registrada no plâncton (23 taxa), seguido de Pistia sp. e Salvinia sp. (18 taxa cada). As famílias encontradas no plâncton foram Difflugiidae, Arcellidae, Centropyxidae, Cryptodifflugiidae, Netzeliidae e Trigonopyxidae, e nas macrófitas foram Difflugiidae, Lesquereusiidae, Netzeliidae e Euglyphidae. Seis espécies constituem primeiras ocorrências para o estado da Bahia: Arcella costata, Ciclopyxis arcelloides, Difflugia dragana, D. gigantea, D. kabylica e D. helvetica. Com o desaparecimento das macrófitas, houve intercambio espacial das espécies Centropyxis hirsuta, D. kabylica e Pentagonia maroccana para o plâncton, evidenciando a plasticidade destes organismos em ocupar ambientes eutrofizados, instáveis e sazonais, tolerando contaminação fecal.
Background: Arthropod-borne viruses have recently emerged and are pathogens of various human diseases, including dengue, zika, and chikungunya viruses.
Methods:We collected Aedes aegypti larvae (N = 20) from Brumado, Bahia, Brazil, and treated and individually preserved the specimens. We analyzed the samples for dengue, zika, and chikungunya viruses using molecular biology methods.
Results:We found that 25% (N = 5) and 15% (N = 3) were positive exclusively for dengue and chikungunya viruses, respectively; 15% (N = 3) were coinfected with both.Conclusions: This is the first report of dengue and chikungunya virus coinfection in A. aegypti larvae.
Testate amoebae are single-celled protists whose protoplasm is contained within a carapace composed of gelatinous to imbricated chitinous plates having variable chemical compositions. We inventoried the testate amoebae community present in the plankton and periphyton of the Pantanal dos Marimbus swamp in Bahia state, Brazil. Sixty-five samples of plankton and periphyton were analyzed during May and September/2014 and April/2015. We identified 65 taxa of testate amoebae distributed in eight families, 15 genera, 45 species, and 16 varieties. The most representative families were Arcellidae, Centropyxidae, and Difflugiidae, corresponding to 24.6%, 19.7% and 14.8% of the taxa respectively. The periphyton had greater richness (59 taxa) than the plankton (39 taxa). Of the taxa found, 15 represent new records for Bahia and 14 for northeastern Brazil. The Marimbus Pantanal swamp showed an expressive richness of testate amoebae, and our data increases the number of species of these organisms known to Bahia and northeastern Brazil, where work with those organisms is still scarce.
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