Winter resources are crucial for wildlife, and, at a local scale, some anthropogenic and environmental factors could affect their availability. In the case of wolves, it is known that vocalisations in response to unfamiliar howls are issued to defend their territory and the important resources within it. Then, we studied the characteristics of winter response sites (WRS) during the cold season, aiming to assess their eventual ability to provide insights into the distribution of valuable resources within their territories. Within this scope, we planned a wolf-howling survey following a standardised approach. The study covered an Apennine (Central Italy) area of 500 km2. A hexagonal mesh was imposed on the area, in order to determine the values of different variables at the local scale. A logistic LASSO regression was performed. WRS were positively related to the presence of thermal refuges (odds = 114.485), to patch richness (odds = 1.153), wild boar drive hunting areas (odds = 1.015), and time elapsed since the last hunt (odds = 1.019). Among negative factors, stray dogs reply considerably affects wolves’ responsiveness (odds = 0.207), where odds are the exponentiated coefficients estimated by the logistic lasso regression. These results suggest that WRS are related to anthropogenic and environmental factors favouring the predation process.

The current study reports an ethnobotanical field investigation of traditionally gathered and consumed wild greens (Chorta) in one of the five so-called Blue Zones in the world: Ikaria Isle, Greece. Through 31 semi-structured interviews, a total of 56 wild green plants were documented along with their culinary uses, linguistic labels, and locally perceived tastes. Most of the gathered greens were described as bitter and associated with members of Asteraceae and Brassicaceae botanical families (31%), while among the top-quoted wild greens, species belonging to these two plant families accounted for 50% of the wild vegetables, which were consumed mostly cooked. Cross-cultural comparison with foraging in other areas of the central-eastern Mediterranean and the Near East demonstrated a remarkable overlapping of Ikarian greens with Cretan and Sicilian, as well as in the prevalence of bitter-tasting botanical genera. Important differences with other wild greens-related food heritage were found, most notably with the Armenian and Kurdish ones, which do not commonly feature many bitter greens. The proven role of extra-oral bitter taste receptors in the modulation of gastric emptying, glucose absorption and crosstalk with microbiota opens new ways of looking at these differences, in particular with regard to possible health implications. The present study is also an important attempt to preserve and document the bio-cultural gastronomic heritage of Chorta as a quintessential part of the Mediterranean diet. The study recommends that nutritionists, food scientists, and historians, as well as policymakers and practitioners, pay the required attention to traditional rural dietary systems as models of sustainable health.

The R packages MaxSkew and MultiSkew measure, test and remove skewness from multivariate data using their third-order standardized moments. Skewness is measured by scalar functions of the third standardized moment matrix. Skewness is tested with either the bootstrap or under normality. Skewness is removed by appropriate linear projections. The packages might be used to recover data features, as for example clusters and outliers. They are also helpful in improving the performances of statistical methods, as for example the Hotelling’s one-sample test. The Iris dataset illustrates the usages of MaxSkew and MultiSkew.

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