Intramuscular injections are administered into deep muscle tissue and can threaten human life if it is applied to the wrong area. Therefore, safe areas should be preferred for injection administration. Purpose: The aim of this study was to evaluate the effect of the training on intramuscular injection to ventrogluteal region given to the senior nursing students on their knowledge, opinions and status of preferring this region . Method: The study is a methodological study with single group and pretest- posttest design. The population of the study was composed of the senior nursing students (N=70) studying in SANKO University. 42 students completed the study. The data were collected with survey method. Results :It was determined that the prejudices of the students about injection to ventrogluteal region changed significantly and positively after the training (90.5%) and their information level increased (p<0.05), but the students had negative thoughts about their self-confidence and about the possible objection of the patients (p>0.05). Students scored the demonstration training as 8 out of 10 (SD=2.03). Conclusion: The study results indicated that the information level of the students about injection into the ventrogluteal region increased and their prejudices changed positively.