Imaging intact human organs from the organ to the cellular scale in three dimensions is a goal of biomedical imaging. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using the European Synchrotron Radiation Facility (ESRF)’s Extremely Brilliant Source (EBS). The spatial coherence of the ESRF-EBS combined with our beamline equipment, sample preparation and scanning developments enabled us to perform non-destructive, three-dimensional (3D) scans with hierarchically increasing resolution at any location in whole human organs. We applied HiP-CT to image five intact human organ types: brain, lung, heart, kidney and spleen. HiP-CT provided a structural overview of each whole organ followed by multiple higher-resolution volumes of interest, capturing organotypic functional units and certain individual specialized cells within intact human organs. We demonstrate the potential applications of HiP-CT through quantification and morphometry of glomeruli in an intact human kidney and identification of regional changes in the tissue architecture in a lung from a deceased donor with coronavirus disease 2019 (COVID-19).

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Short 'product lives' and disposable packaging result in premature disposal of valuable resources. Industrialisation, mass production and global supply chains have resulted in a disconnect between people, places, materials and design. Upcycling is reuse of discarded materials which results in an increase in 'value'. We discuss the potential for creative upcycling to reconnect people with materials and establish cultures and communities of making. The reaction of the public to creative reuse is explored by creating a café structure made entirely from recycled materials, and this provides the starting point to consider the contexts in which upcycling occurs, the motivations for (and barriers to) reuse and upcycling, the potential benefits of upcycling in the context of affluent Western 'consumer' societies, and the scope for designers to imbue objects with the potential for creative reuse. We argue that designing to enable creative upcycling allows the future lives of objects to be contingent on context and culture, rather than being prescribed by the designer, with potential for widespread social, economic and environmental benefits.