In this work, we analysed the effect of aguamiel (sap from Agave salmiana) on haematic biometry in rabbits as well as its antioxidant activity with the objective of determining its nutritional value. Nine male Landrace 55-day-old rabbits were sorted into three groups of three rabbits each, which had access to fresh aguamiel, boiled aguamiel and water instead of aguamiel. Commercial rabbit food was supplied ad libitum to each experimental unit. Fresh aguamiel promoted weight increases of about 13% by the seventh week. Haemoglobin counts increased by 4.5% and 9% when rabbits were fed with fresh and boiled aguamiel, respectively. Haematocrit counts increased from 2.6% to 5.3%. Mean corpuscular volume did not show a change. Mean corpuscular haemoglobin significantly increased up to 12% above that previously reported on rabbits. Observed Fe (fixed to transferrin), transferrin and ferritin content increased slightly when fresh aguamiel was included in the diet, as compared to the control. In bone, Fe increased 79.3% (boiled aguamiel) while P increased 23.6% (fresh aguamiel). In antioxidant activity determination, the aguamiel showed 20% with respect to that found in coffee and/or grape juice (commercial beverages with a high antioxidant capability), 226.2 µmol gallic acid equivalent and 35.1 mg ascorbic acid in 250 mL, respectively. These results showed that aguamiel is an innocuous beverage to feed animals, conferring some beneficial properties, and it could be considered as a nutraceutical product

Honeywater or aguamiel (Agave sap) has been consumed by Mexican population since pre-columbian times. Although, it has been claimed by folk belief that aguamiel possesses some medicinal properties, scientific studies on its effect on human health have not been well documented. The behavior of blood components in nine volunteers (two young males, three adult females and four adult males) after aguamiel consumption (250 mL every three days during a period of 35 days) was analyzed. It was found that, serum red blood cell count, serum white blood cell count, platelet count, minerals (Zn, Mg and Fe) and iron-related proteins (ferritin and transferrin) levels were not negatively affected because of all of these blood indicators ranged within normal reference values. However, this study showed that aguamiel presented a specific functional effect since hypercholesterolemic adult males showed normal levels of serum total cholesterol after aguamiel consumption, whereas total cholesterol levels were kept in normal ranges after aguamiel consumption for normocholesterolemic subjects. Furthermore, aguamiel consumption did not cause hyperglycemia in any of the tested groups.