Pseudomonas aeruginosa (P. aeruginosa) has been found to be a common hospital acquired pathogen, responsible for several severe infections. The objective of this study was to describe the antibiotic resistance profile of P. aeruginosa strains from human sample. This descriptive study was carried out on 168 isolated strains of P. aeruginosa collected from January 2014 to December 2015 at the Pasteur Institute of Côte d'Ivoire. The strains identification was done according to the methods of conventional bacteriology. The antibiotic sensitivity tests were performed using the disc diffusion method in agar medium according to CA-SFM (Antibiogram committee of French society of microbiology) criteria. The serotyping of the strains was carried out by using the agglutination method slide test, with the aid of 4 specific polyvalent antisera. The most prevalent P. aeruginosa serotypes were O4 (24.4); O11 (14.6); and O6 (9.5%). The rate of antibiotic resistance to ticarcillin was 32.9%, ciprofloxacin 18.4%, ceftazidime 14.9%, Imipenem 11.3%, and amikacin 11.3%. Resistance to Imipenem was above 10% in an intensive care unit and in the pneumonology unit (PPH). Strains of O6 serotypes were the most multidrugresistant followed by O11 with respective rates of 31.2 and 28% MDR (Multidrug Resistance). P. aeruginsa are microorganism capable of developing mechanisms of complex resistance which makes it difficult to manage. The attention of hygiene rules and the rational use of antibiotics are very important in other to prevent the spread of MDR P. aeruginosa.