Introduction It has been demonstrated that testing conditions may influence sexual performance in many mammals, including male rats. We recently developed a multiple partner choice arena (MPCA) consisting of four acrylic cylinders placed in a cross pattern with one male in each cylinder. A sexually receptive female rat was introduced into the center of the MPCA and was allowed to choose a male to copulate with. The female showed a preference for one of the four males, remaining longer and copulating more times with it. Aim The study aims to evaluate and compare the copulatory pattern of male rats in two arenas: the standard arena (SA) and the MPCA. Methods In Experiment 1, a group of 10 male rats mated in an SA (a closed cylinder) and 2 weeks later they mated in the MPCA, in order to compare different parameters of male sexual behavior. In Experiment 2, the sexual behavior of two different groups of sexually experienced male rats was tested in two conditions: the SA and the MPCA. In the latter, only the behavior of the preferred (P) males and nonpreferred (NP) males that ejaculated was recorded. Main Outcome Measures The main outcome is the number of intromissions preceding ejaculation and the latencies to mount, intromit, and ejaculate. Results In Experiment 1, the number of intromissions was significantly reduced and the intromission and ejaculation latencies were significantly shortened when the males were tested in the MPCA rather than in the SA. In Experiment 2, both groups of males tested in the MPCA (P and NP) showed a significant reduction in the number of intromissions preceding ejaculation and shorter mounting and ejaculation latencies in comparison with rats in the SA. This decrease was more noticeable in NP males. Conclusions The MPCA reduce significantly the ejaculatory pattern in male rats.