Understanding and detecting diseases of amphibians has become vitally important in conservation and ecological studies and prevent and biosecurity a determinant priority in experimental farms, mainly when related with academic and research activities. Ranavirus belongs to the family Iridoviridae, and causes an emergent infectious disease that affects different species, especially fish, reptiles and amphibians, with a significant contribution to the decline of the population. In amphibian systems, Ranaviruses transmission can occur between vertebrate classes through direct contact, by scavenging or through virus particles persisting in the environment. Subclinical infected individuals may serve as reservoirs in the most susceptible anura species. Humans play a significant role in this emergent disease and biosecurity measures are determinant to prevent the introduction of these viruses, either in commercial or experimental farms. A Biosafety Plan is a fundamental tool in the Ranaviruses prevention and include educational and training programs, relevant to the mission of a Higher Education Institution.