AgSIE was used for the direct analysis of folic acid (FA), with a detection limit and lower level of quantitation of 6.8×10−10 mol L−1 and 2.3×10−8 mol L−1. The analysis in fresh and processed fruits was done without any sample pretreatment. In strawberry and acerola juices, FA concentration level values were below the method detection limit. FA was detectable in peach (77.7±0.4 µg L−1 and 64.4±0.5 µg L−1), Persian lime (45.4±0.7 µg L−1), pineapple Hawaii (66.2±0.4 µg L−1), pear pineapple (35.3±0.6 µg L−1), cashew (54.4±0.5 µg L−1), passion fruit (73.2±0.3 µg L−1), and apple (84.4±0.5 µg L−1).