We used two methods to estimate short-wave (S) cone spectral sensitivity. Firstly, we measured S-cone thresholds centrally and peripherally in five trichromats, and in three blue-cone monochromats, who lack functioning middle-wave (M) and long-wave (L) cones. Secondly, we analyzed standard color-matching data. Both methods yielded equivalent results, on the basis of which we propose new S-cone spectral sensitivity functions. At short and middle-wavelengths, our measurements are consistent with the color matching data of Stiles and Burch (1955, Optica Acta, 2, 168-181; 1959, Optica Acta, 6, 1-26), and other psychophysically measured functions, such as pi 3 (Stiles, 1953, Coloquio sobre problemas opticos de la vision, 1, 65-103). At longer wavelengths, S-cone sensitivity has previously been over-estimated.

Hue shifts were measured in isoluminant color gratings whose bar width was varied from 2' to 20' ofvisual angle. Subjects matched the hues in each grating with individual Munsell swatches. Hue shifts were largest for bar widths of 2'; however, they depended on the color combination used. Green and red shifted toward (i.e., assimilated with) whatever second grating color they were paired with. Blue, on the other hand, assimilated with red and with yellow, but remained relatively unchanged when combined with green. Yellow shifted only minimally, regardless of the second grating color. Hue shifts decreased with increasing stripe width and disappeared between 4.5' and 7.5'. Compared with the assimilative hue shifts, color contrast effects were slight or absent. These results cannot be attributed merely to chromatic aberration, macular pigment, eye movements, or field size.

SUMMARY1. Incremental thresholds were measured in a retinal region 12 deg temporal from the fovea with a target of 200 ms in duration and 6 deg in diameter superimposed on background fields of various intensities and wavelengths. Measurements were made under rod-isolation conditions in five normal observers and in a typical, complete achromat observer who had no cone function.2. The rise in threshold with background intensity changes with background wavelength in the normal trichromat observers. On 450, 520 and 560 nm backgrounds the average slope in logarithmic co-ordinates (0-78+0-04, S.D.) is similar to that found for the achromat -whose slope is independent of background wavelength (0 79 +003) -but on a 640 nm background it more nearly approaches Weber's law (0 91 + 0 02). This indicates that the sensitivity of the rods to an incremental target is not determined by quantal absorptions in the rods alone but by quantal absorptions in both the rods and the cones.3. Rod incremental thresholds were also measured in various colour-blind observers lacking one or more of the cone classes: a blue-cone monochromat, four deuteranopes and a protanope. For the blue-cone monochromat, like the achromat, the slope of the increment threshold curve is constant with background wavelength. For the deuteranopes and the protanope, like the normal, the slope increases with wavelength. The protanope, however, shows a smaller increase in slope, consistent with the lower sensitivity of his cones to long-wavelength light.4. The dependence of the field adaptation of the rods on the cones was confirmed by field-mixture experiments, in which the incremental threshold was measured against bichromatic backgrounds, and in silent substitution experiments, in which backgrounds equated for their effects on either the cones or the rods but not both were instantaneously substituted for one another. INTRODUCTIONThe visual system adapts to variations in ambient illumination by adjusting its sensitivity. This change in sensitivity is typically monitored by measuring the change in detection threshold for a small target flash as a function of the intensity of a larger adapting background (Stiles, 1939

The incremental threshold of the isolated rod visual system is believed, under certain conditions, to obey Weber's law (that is, to increase in direct proportion to the intensity of the background). This relation was tested at several background wavelengths, over an intensity range for which the target was seen only by the rods. Although the slope on long-wavelength background approximates unity (that is, Weber's law on log-log coordinates), it averages less than 0.8 on short- and middle-wavelength backgrounds. This is the same value as that found for the thresholds of a typical, complete achromat--who lacks cone vision--regardless of background wavelength. These results force the conclusion that Weber's law for incremental threshold detection is achieved not by the rods alone but only by the rods acting together with the cones.

SUMMARY1. Absolute and increment thresholds were measured in a retinal region 12 deg temporal from the fovea with 520 nm targets of varying size and duration. Measurements were made under rod-isolation conditions in two normal observers and in a typical, complete achromat observer who has no cone-mediated vision. The purpose of these experiments was to determine how the temporal and spatial summation of rod-mediated vision changes with light adaptation.2. The absolute threshold and the rise in increment threshold with background intensity depend upon target size and duration, but the psychophysically estimated dark light of the eye (the hypothetical light assumed to be equivalent to photoreceptor noise) does not.3. The rise in increment threshold for tiny (10 min of arc), brief (10 ms) targets approaches the de Vries-Rose square-root law, varying according to the quantal fluctuations of the background light. The slope of the rod increment threshold versus background intensity (TVI) curves in logarithmic co-ordinates is about 0-56+0-04 (when cones are not influencing rod field adaptation). For large (6 deg) and long (200 ms) targets, a maximum slope of about 0 77 + 0 03 is attained.4. The steeper slopes of the rod-detected TVI curves for large, long targets implies some reduction in temporal or spatial summation. In fact, the change in summation area is much more critical: under conditions where only the rod system is active the TVI curve slope is independent of target duration, suggesting that temporal summation is practically independent of background intensity.5. The rise in threshold also depends on the wavelength of the background field in the normal observer but not in the achromat, confirming reports that the field adaptation of the rods is not independent of the quantal absorptions in the cones. The cone influence is most conspicuous on long-wavelength backgrounds and is found for all target sizes and durations, but is greater for large and long targets than for the other conditions. MS 9972