Abstractobjective To evaluate combinations of larvicides and fish which are predators of larvae of Aedes aegypti, namely Betta splendens, Trichogaster trichopterus and Poecilia reticulata.methods We used 15 water tanks with a capacity of 250 litres of water. In 10 tanks, larvicideTemephos, Bti and Novaluron -was added, the other five contained only one specimen of fish and unchlorinated water. The fish were monitored for a week without changing the water, and their survival recorded on a form. An estimate of the Kaplan-Meier survival was performed to determine the significance of the tests.results Betta splendens showed the lowest mortality range of larvicides tested. conclusion Combined use of larvivorous fish and larvicides in large water tanks is feasible.

This study aimed to identify the seroprevalence of chikungunya and its associated factors in the city of Quixadá, Ceará, Brazil. We also aimed to identify the spatial distribution patterns of positive cases. A cross-sectional survey was conducted with a questionnaire about clinical symptoms, socioeconomic and demographic factors, and a 10 ml blood sample was collected and analysed by ELISA. For the bivariate analysis, we use the chi-square test, a prevalence ratio and its 95% confidence interval. A robust Poisson hierarchical regression was used to adjust for confounders. The Kernel density was performed for the spatial analysis. A total of 409 samples were analysed; of them, 70.7% were seropositive for previous exposure to chikungunya virus (CHIKV). High seropositivity for CHIKV was higher in female participants (75.5%;