In ground nesting upland birds, reproductive activities contribute to elevated predation risk, so females presumably use multiple strategies to ensure nest success. Identification of drivers reducing predation risk has primarily focused on evaluating vegetative conditions at nest sites, but behavioral decisions manifested through movements during incubation may be additional drivers of nest survival. However, our understanding of how movements during incubation impact nest survival is limited for most ground nesting birds. Using GPS data collected from female Eastern Wild Turkeys (n = 206), we evaluated nest survival as it relates to movement behaviors during incubation, including recess frequency, distance traveled during recesses, and habitat selection during recess movements. We identified 9,361 movements off nests and 6,529 recess events based on approximately 62,065 hr of incubation data, and estimated mean nest attentiveness of 84.0%. The numbers of recesses taken daily were variable across females (range: 1‒7). Nest survival modeling indicated that increased cumulative distance moved during recesses each day was the primary driver of positive daily nest survival. Our results suggest behavioral decisions are influencing trade‐offs between nest survival and adult female survival during incubation to reduce predation risk, specifically through adjustments to distances traveled during recesses.

Northern bobwhites (Colinus virginianus) are small quails in the New World Quail family (Odontophoridae) and are one of the most phenotypically diverse avian species. Despite extensive research on bobwhite ecology, genomic studies investigating the evolution of phenotypic diversity in this species are lacking. Here, we present a new, highly contiguous assembly for bobwhites using tissue samples from a vouchered, wild, female bird collected in Louisiana. By performing a de novo assembly and scaffolding the assembly with Dovetail Chicago and HiC libraries and the HiRise pipeline, we produced an 866.8 Mb assembly including 1,512 scaffolds with a scaffold N50 of 66.8 Mb, a scaffold L90 of 17, and a BUSCO completeness score of 90.8%. This new assembly represents approximately 96% of the non-repetitive and 84% of the entire bobwhite genome size, greatly improves scaffold lengths and contiguity compared to an existing draft bobwhite genome, and provides an important tool for future studies of evolutionary and functional genomics in bobwhites.

Wild turkey nest success is variable spatially and temporally, with predation being the primary driver of nest loss. Historically, characteristics of nest site vegetation have been used to predict the probability of a nest being successful, a corollary to not being preyed upon. Implicit in nest success studies is that the density, distribution, and habitat preferences of predator species, relative to wild turkey nest distribution, drive the predation risk of nests on the landscape. We quantified diversity of wild turkey nest predator species within 11-ha incubation ranges centered on active nest sites, scent baited passive nest sites, and scent baited and unbaited random sites and developed a spatially explicit predictive model for potential nest predator distribution based on vegetation and landform characteristics that occurred within incubation ranges. We used 210 camera sites on passive (nesting area from previous year), active (nesting area same year), and random sites over 5,144 trap nights (TN) and recorded 2,925 photographs of potential nest predator species. Relative abundance indices (RAI) indicated greater abundance in passive (14.2/100 TN) and active (12.7/100 TN) nesting sites relative to random sites (3.13/100 TN). For active nesting sites, successful nests had lower RAI (1.8/100 TN) than unsuccessful nests (10.4/100 TN) and lower RAI than baited and unbaited random sites (2/100 TN and 4.3/100 TN, respectively).Our results indicate that potential wild turkey nest predator species regularly occurred within incubation ranges, but were more likely to occur in locations with nests than in random locations on the landscape. We did not document a strong

21 22 Northern bobwhites (Colinus virginianus) are small quails in the New World Quail family 23 (Odontophoridae) and are one of the most phenotypically diverse avian species. Despite 24 extensive research on bobwhite ecology, genomic studies investigating the evolution of 25 phenotypic diversity in this species are lacking. Here, we present a new, highly contiguous 26 assembly for bobwhites using tissue samples from a vouchered, wild, female bird collected in 27 Louisiana. Using Dovetail Chicago and HiC libraries with the HiRise assembly pipeline, we 28 produced an 866.8 Mbp assembly including 1,512 scaffolds with a contig N50 of 66.8 Mbp, a 29 scaffold L50 of four, and a BUSCO completeness score of 90.8%. This new assembly greatly 30 improves scaffold lengths and contiguity compared to an existing draft bobwhite genome and 31 provides an important tool for future studies of evolutionary and functional genomics in 32 bobwhites. 33