This study characterised Escherichia coli isolated from Creek Road/Bonny Estuary using molecular techniques. One hundred and twenty (120) water samples were investigated for the presence of E. coli in Creek Road/Bonny Estuary. Most Probable Number (MPN) Technique, Eijkman test and molecular techniques were used for the isolation and detection of E. coli. The analysis was carried out periodically (morning and evening) and seasonally (end of rainy season (November), the start of rainy season (April) and in the middle of rainy season (July)). All E. coli isolates were screened for resistance genes in the pathotypes of E. coli (EHEC, EPEC, EAEC, ETEC and EIEC), including Sulfhydryl Variable (SHV), CTX-M, Temoniera (TEM) and MCR genes using the Polymerase chain reaction Technique. The results showed that 100% of the water samples were positive for coliforms and all the water samples harboured E. coli. The highest total coliforms count recorded was in the morning of April (48 MPN/100 ml). The distribution of individual genes in the E. coli isolates are: SHV (5.0), CTX-M (5.8), TEM (4.2), MCR (0.0%), stx1 and stx2 (4.2), esV and bfA (1.7), aaiC (4.2), elt (3.3) and invE (2.5). The CTX-M was the most found of all the resistance genes.

The bacteriological quality of swimming pool water in Port Harcourt Metropolis was investigated. Ten (10) swimming pools were examined for microbial quality. Out of the 10 swimming pools, 4 (2, 4, 7 and 9) had bacterial isolates of 40 (100%). Swimming pools 2, 4, 7 and 9 had the bacterial isolates of 10 (25%), 8 (20%), 10 (25%) and 12 (30%), respectively. Of the forty (40) bacterial isolates identified, which represented 100 percent, 22 (55%) were identified as Staphylococcus epidermidis, 10 (25%) Bacillus cereus, 6 (16%) Micrococcus and 2 (5%) Staphylococcus aureus. Among the four swimming pools, 2 and 9 did not have the isolates of Staphylococcus aureus and Micrococcus, respectively. Based on the World Health Organisation (WHO) standard for recreational waters, the absence of coliform and fecal coliform bacteria (E. coli) revealed that the ten (10) swimming pools used for this study are considered to be within the acceptable limits for certifying microbiological water quality. However, there is a need for care and continuous maintenance of the swimming pools.

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This study was carried out to assess the Physicochemical and Bacteriological qualities of borehole water sources in Gokana Local Government Area, Rivers State, Nigeria. Twenty (20) boreholes were used for the study and a total of sixty (60) water samples were collected for bacteriological analyses. All the Physicochemical parameters were measured in situ using Horiba Water Checker (Model-10). Heterotrophic Plate Count, Most Probable Number technique and Eijkman test were used for the enumeration, isolation and identification of bacteria. All analyses for Physicochemical parameters were within the acceptable limits (pH: 6.3 to 7.7; Temperature: 27 to 30; Turbidity: 0.61 to 2.01). Bacteriological quality was higher than the World Health Organisation standard recommended limits. The detection of bacteria such as Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Proteus and Klebsiella species in the borehole water sources that are intended for human consumption could cause one to think that water from those sources may lead to severe health risks to consumers. Hence, it is not suitable for direct human consumption without treatment. Therefore, it is recommended that onsite treatment intervention be mobilised in order to protect residents of Gokana LGA of Rivers State, Nigeria from further possible consequences of using the borehole water sources.

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