Clinical applications of precision oncology require accurate tests that can distinguish true cancer specific mutations from errors introduced at each step of next-generation sequencing (NGS). To date, no bulk sequencing study has addressed the effects of cross-site reproducibility, nor the biological, technical and computational factors that influence variant identification. Here we report a systematic interrogation of somatic mutations in paired tumor-normal cell lines to identify factors affecting detection reproducibility and accuracy at six different centers. Using whole genome sequencing (WGS) and whole-exome sequencing (WES), we evaluated the reproducibility of different sample types with varying input amount and tumor purity, and multiple library construction protocols, followed by processing with nine bioinformatics pipelines. We found that read coverage and callers affected both WGS and WES reproducibility, but WES performance was influenced by insert fragment size, genomic copy content and the global imbalance score (GIV; G > T/C > A). Finally, taking into account library preparation protocol, tumor content, read coverage and bioinformatics processes concomitantly, we recommend actionable practices to improve the reproducibility and accuracy of NGS experiments for cancer mutation detection.

The opacity and mundaneness of racism often allows it to slip through our traditional systems of accounting and measuring. The study of racialized emotions has been an important intervention in sociology to understand the intimate nature of racialized social structures. There still is a need to understand the language Black communities use to communicate their complex emotional worlds and the nuanced ways abusive power systems are felt in everyday life. Using 24 months of ethnographic fieldwork in northeast North Carolina and data from 23 in-depth interviews, the author examines the relationship between Black people’s emotional habitus and racial structures. The results indicate that Black people developed vibe as a rhetorical tool to articulate their complex emotional economy and it is regularly used to make sense of racialize experiences. Vibe is not limited to racial understanding as it works to name the often unsayable and perceptive ways people know, feel, and respond to the opacity and non-quantifiable dimensions of social experience. This paper focuses on the ways Black community members used vibe to articulate feeling the criminalization of Blackness or what this research refers to as ‘felt criminality.’ Despite facing emotional subjugation Black community members were still invested in emotive projects and used their felt experience as an epistemological resource to make sense of racial processes in a supposedly colorblind society.

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