A group of black-legged mosquito species within the subgenus Ochlerotatus Lynch Arribalzaga, 1891 of the genus Aedes Meigen, 1818 is difficult to identify based on their morphology. This group includes Aedes pionips Dyar, 1919, whose first record in the Erzgebirge (Germany) is reported in this study. The objective was to compile and add species specific characters for the morphological discrimination of Ae. pionips from similar mosquitoes in order to facilitate species identification. Generated cytochrome c oxidase subunit I gene DNA sequences of Ae. pionips from Germany were compared to sequences from related species, using a phylogenetic tree.