Background: Palliative care and spirituality are still very little discussed, but when studied and applied they become capable of alleviating some of the dimensions of suffering of man.Objective: To evaluate the understanding of health professionals about the concept of assistance to patients under palliative care and spirituality, as well as the application of techniques to alleviate the spiritual suffering for these patients.Method: This study used questionnaires in 91 professionals that work in the palliative care area (doctors, nurses and psychologists, chaplains), with at least three years of experience, regardless of gender. Results:The study shows a divergence in the work of professionals of the multidisciplinary staff, as well as the difficulty in addressing issues of spirituality. Conclusion:The palliative care and spirituality provide relief to many suffering patients, it is important to have health professionals aligned in concepts and prepared to use it in clinical practice.