No abstract

Deuterium-depleted water (DDW) has a concentration of deuterium 6-7 times lower than naturally occurring water (20-25 ppm vs. 150 ppm). When administered for a longer period, it can reduce the concentration of deuterium throughout the body, activating cellular mechanisms that depend on protons. The aim of the present work was to investigate the influence of chronic DDW administration on anxiety-related processes in Wistar rats when compared to a control group that received distilled water, as studied in an elevated plus maze behavioral test. Our results describe a possible anxiolytic-like effect of DDW administration on rats, as shown by the increase in the percentage of time and number of entries in the open arms of the elevated plus maze. The administration of DDW also resulted in stimulated head-dipping behavior in the open arms, which is a behavioral change that characterizes the exploratory behavior and decreased inhibition/ fear in an unfamiliar environment. We conclude that the change in this balance may have important consequences for many biological mechanisms. A deuterium desaturation treatment with DDW might have a use in anxiety disorders.

A series of experiments have shown that deuterium depleted water has beneficial effects for life. The deuterium concentration interest area for deuterium depleted water is in the range 20-110 ppm. Deuterium depleted water is obtained by isotopic distillation under vacuum. Advanced studies have been conducted on the process of obtaining deuterium depleted water by isotopic distillation process. Deuterium concentration in deuterium depleted water was analyzed by mass spectrometry.