Hypertension is often characterised by impaired vasodilation involving dysfunction of multiple vasodilatory mechanisms. ω‐3 polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) can reduce blood pressure and vasodilation. In the endothelium, DHA and EPA improve function including increased NO bioavailability. However, animal studies show that DHA‐ and EPA‐mediated vasodilation persists after endothelial removal, indicating a role for vascular smooth muscle cells (VSMCs). The vasodilatory effects of ω‐3 PUFAs on VSMCs are mediated via opening of large conductance calcium‐activated potassium channels (BKCa), ATP‐sensitive potassium channels (KATP) and possibly members of the Kv7 family of voltage‐activated potassium channels, resulting in hyperpolarisation and relaxation. ω‐3 PUFA actions on BKCa and voltage‐gated ion channels involve electrostatic interactions that are dependent on the polyunsaturated acyl tail, cis‐geometry of these double bonds and negative charge of the carboxyl headgroup. This suggests structural manipulation of ω‐3 PUFA could generate novel, targeted, therapeutic leads.

DNA nanotechnology allows for the creation of membrane-spanning channels with customized shape and functionality. We present three novel designs with channel diameters spanning an order of magnitude from 0.8 nm to 8 nm. We utilize DNA tile assembly and scaffolded origami with two different scaffold lengths to create channels of variable size and architectural complexity. Bifunctional porphyrin-and cholesterol-tags serve as membrane anchors to facilitate insertion into the lipid membrane (J. R. Burns, K. Göpfrich et al., Angewandte Chemie, 2015). We compare the conductance of the channels and confirm the correspondence between engineered design and single-channel behaviour. Our channels span two orders of magnitude in conductance, comparable to protein pores encompassing small ion channels as well as large porins. Conductance states are dependent on transmembrane voltage (K. Göpfrich, A. Seifert, et al., ACS Nano, 2014). We demonstrate that self-assembly and membrane attachment of simple DNA channels can be achieved within a minute, making their creation scalable for applications in biology (K. Göpfrich et al., Nanoletters, 2015). Confocal imaging with ion-sensitive dyes serves as an independent proof of the cation-selective ion transport capabilities of our DNA channels. Our work showcases the versatility of artificial DNA-based pores inspired by the rich structural and functional diversity of natural membrane components.