The aim of the study is to investigate the methicillin-resistant Staphylococcus spp. nasal colonization among dental surgeon professors. Dental surgeon professors of a Higher Education Institution (HEI) responded to a questionnaire covering sociodemographic, employment and behavioral data, and were subjected to clinical specimen collection by nasal swab. Identification and susceptibility testing of bacteria were performed by automated method (Vitek 2 compact TM ). Susceptibility to mupirocin was tested by disk-diffusion method. The detection of mecA and lukS-F genes was performed by PCR. The genetic similarity among the isolates was determined by Pulsed Field Gel Electrophoresis. Four (9.7%) dental surgeon professors were colonized by methicillin-resistant Staphylococcus spp. and claim have provided care to patients without wearing surgical masks (1/4) and/or gloves (4/4), and had the habit of keeping surgical masks on the chin (1/4). Two S. aureus and one S. epidermidis isolates were mecA gene positives. MLS B complex (inducible), mupirocin and sulfamethoxazole/trimethoprim resistance were also detected. The lukS-F gene was not detected in any S. aureus and no genetic similarity was found among the isolates. Dental surgeon professors were found to be colonized with methicillinresistant Staphylococcus spp. and declared noncompliance to infection control practices, posing risk of infection to themselves, patients, students and their families.