Purpose The aim of this study was to investigate protozoan cysts and oocyts in the faecal pellets of the German cockroach ( Blattella germanica ), with emphasis on the prevalence of Lophomonas blattarum . Methods Thirty adult Blattella germanica (12 males; 18 females) were trapped near Madrid, Spain. Expelled faecal pellets were collected in the laboratory over 5 days in two plastic containers. Protozoan cysts from one container were weighted and those in the other used for morphologically identification. Results Protozoan cysts/oocysts per gram of faecal pellet were: Nyctotherus sp. (0.0019/g), Entamoeba (0.0007/g), Balantidium coli (0.0001/g), Lophomonas blattarum (0.00038/g). Observation of 189 protozoan cysts/oocysts indicated the following prevalence: Nyctotherus sp. 65 (34.4%); Gregarina spp. 34 (18%); Entamoeba sp. 24 (12.7%); Cryptosporidium sp. 17 (9%); Coccidia 16 (8.4%); Lophomonas blattarum 13 (6.8%); Balantidium coli 4 (2.1%); and unclassified 16 (8.4%). Conclusion Lophomonas blattarum has previously been demonstrated in the gut of cockroaches, but this is the first assessment of the prevalence in Blattella germanica in faecal pellets. The presence of protozoa in faecal pellets provides some evidence for one step in a hypothesised route of respiratory infection whereby protozoa from household insects enter the respiratory tract.

The German cockroach (Blattella germanica) is a common domestic pest, which produces allergens that have been associated with broncho-pulmonary disease. Various protozoan species have been identified in the intestine of this cockroach and it has been hypothesised that these protozoa, or their proteases, may contribute to the burden of cockroach-associated allergens and adjuvants present in domestic dust. The aim of this study was therefore to determine the prevalence of protozoan species in the intestine of Blattella germanica. German cockroaches were anesthetised and dissected and gut contents are used to produce wet slides for microscopy. Both, Giemsa and Papanicolaou stains were used to confirm correct identification of Lophomonas blattarum. Representatives of four genera of protozoa were identified in 110 cockroaches: Nyctoterus sp. was observed in 91.8% of cases, Gregarina sp. in 64.5%, Amoeba sp. in 25.4% and Lophomonas blattarum in 13.6%. Nyctoterus and Gregarina were statistically significantly more likely to be found in diseased cockroaches compared to Amoeba or Lophomonas. The prevalence of Lophomonas blattarum was similar to that in published studies of a different species of cockroach, Periplaneta americana. Further work is needed to assess the interplay between protozoa, cockroaches and broncho-pulmonary diseases.

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Bacterial vaginosis (BV) is the most common vaginal infection among women of childbearing age. Although the aetiology and pathogenesis of BV remain a matter of some controversy, it appears that the normal protective vaginal lactobacilli are replaced by anaerobic microorganisms. BV can have serious implications, including adverse pregnancy outcomes, increased susceptibility to sexually transmitted infections and infertility. 1 The Papanicolaou method is commonly used for diagnosing BV, providing an alternative to Gram-staining of smears, 2 and is capable of detecting BV in the majority of infected women, both symptomatic and asymptomatic. 3 For several years, we have occasionally observed the presence of intriguing structures similar to "ground-glass-like" globules in cervicovaginal (CV) smears from patients with BV stained using the Papanicolaou (Pap) method, which, to our knowledge, have not previously been reported in CV smears. Thus, the aims of this Correspondence were to provide examples of the appearance of these globules, describe the frequency with which the structures were observed in CV smears, explore their significance, and question whether these globules are related to clinical factors such as age, presence of symptoms, the utilisation of any contraceptive method, or pregnancy. A retrospective analysis was undertaken of all conventional CV smears submitted to a cytology laboratory in Spain over a 2-year period (2013-2014). All the smears had been stained using the Pap method. The percentage of specimens in which globules were observed under a light microscope was determined. A subset of nine positive smears for BV, which demonstrated the presence of globules, were chosen and re-stained with PAS, Masson's trichrome and Gram stain.The clinical information for the patients' whose smears contained globules was reviewed to establish whether there was any correlation between the presence of globules in the Pap smears and BV.Four characteristics were taken into account: age, clinical symptoms, contraceptive use and pregnancy.A total of 39 637 CV smears were submitted to the laboratory over a 2-year period. Of these, 18 slides were positive for the presence of these unusual globules (0.045%). The globules appeared as round structures (20-149 lm in diameter), with smooth and regular borders, a blue-greyish tonality, and with numerous coccobacilli adhering to their edges and covering their surfaces ( Figure 1A, B). A clear association was observed between the existence of globules and BV (18 cases; 1.15%). The difference between two proportions 0/38 123 vs 18/1514 is statistically significant at P<.001 (McNemar's test for difference between two proportions, Innersoft CAD v2.9; Middlesex University, London, UK). The median age of patients with BV was 37.3 years (range 17-62). No statistical differences were found with regards to age and the presence of globules. Of the patients with BV, 877 (57.92%) had CORRESPONDENCE | 241

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