The free radical polymerization of styrene was initiated with azobis(isobutyronitrile) in the presence of benzene sulfonyl chloride. Analysis of the terminal structures of the obtained polystyrene with 1 H NMR spectroscopy revealed the presence of a phenylsulfonyl group at the α-end and a chlorine atom at the ω-end of each polystyrene chain. The terminal chlorine atom in the polystyrene chains was further confirmed through atom transfer radical polymerization (ATRP) of styrene and methyl acrylate using the obtained polystyrenes as macroinitiators and CuCl/2,2'-bipyridine as the catalyst system. GPC traces of the products obtained in ATRP at different reaction times were clearly shifted to higher molecular weight direction, indicating that nearly all the macroinitiator chains initiated ATRP of the second monomers. In addition, the number-average molecular weights of the polystyrenes increased directly proportional to the monomer conversions, and agreed well with the theoretical ones.

D301 resin was used to separate and purify total anthraquinones in Radix et Rhizoma Rhei. It was found that the optimized dynamic adsorption conditions is pH7.0, flow velocity of 1.0 BV/h and extracting solution with concentration of 0.3g/mL and optimized dynamic elution conditions is hydrochloric acid with concentration of 0.15mol/L, 75% ethanol, and flow velocity of 1.0 BV/h. Under the above conditions,the average adsorption rate and desorption rate are respectively 89.75% and 91.15%, indicating that extraction efficiency of total anthracenone of emodin is relatively high and that the technology enjoys strong stability.