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In this paper, we propose a nonmonotone adaptive trust-region method for solving symmetric nonlinear equations problems. The convergent result of the presented method will be established under favorable conditions. Numerical results are reported.

This study aimed to explore the effects of dominant and compliant personalities, on both flow experience and the external characteristics of flow experience. A total of 48 participants were recruited to play an online game and subsequently asked to recall the songs they had heard while they were playing the game. Eye blink rate was recorded. The results demonstrated that (1) the participant was immersed in the game more if he/she was relatively dominant or noncompliant; (2) the perceptions about the external environment declined remarkably while being in a flow state; and (3) eye blink rates decreased only when the flow happened at the beginning of the game, rather than throughout the whole process. The results suggested that gamers who tend to be dominant or noncompliant were more likely to experience flow. Eye blink rate and perceptions of the external environment could be objective indicators of flow experience.

H9N2 subtype avian influenza viruses are widespread in domestic poultry, and vaccination remains the most effective way to protect the chicken population from avian influenza pandemics. Currently, egg-based H9N2 influenza vaccine production has several disadvantages and mammalian MDCK cells are being investigated as candidates for influenza vaccine production. However, little research has been conducted on low pathogenic avian influenza viruses (LPAIV) such as H9N2 replicating in mammalian cells using microcarrier beads in a bioreactor. In this study, we present a systematic analysis of a safe H9N2 influenza vaccine derived from MDCK cells for protecting chickens against influenza virus infection. In 2008, we isolated two novel H9N2 influenza viruses from chickens raised in southern China, and these H9N2 viruses were adapted to MDCK cells. The H9N2 virus was produced in MDCK cells in a scalable bioreactor, purified, inactivated, and investigated for use as a vaccine. The MDCK-derived H9N2 vaccine was able to induce high titers of neutralizing antibodies in chickens of different ages. Histopathological examination, direct immunofluorescence, HI assay, CD4(+)/CD8(+) ratio test, and cytokine evaluation indicated that the MDCK-derived H9N2 vaccine evoked a rapid and effective immune response to protect chickens from influenza infection. High titers of H9N2-specific antibodies were maintained in chickens for 5 months, and the MDCK-derived H9N2 vaccine had no effects on chicken growth. The use of MDCK cells in bioreactors for LPAIV vaccine production is an attractive option to prevent outbreaks of LPAIV in poultry.