In the presented paper, the impacts of steel fiber use and tensile reinforcement ratio on shear behavior of Ultra-High Performance Concrete (UHPC) beams were investigated from the point of different tensile reinforcement ratios. In the scope of the experimental program, a total of eight beams consisting of four reinforcement ratios representing low to high ratios ranged from 0.8% to 2.2% were casted without shear reinforcement and subjected to the four-point loading test. While half of the test beams included 30 mm end-hooked steel fibers (SF-UHPC) with 2.0 vol%, the remaining beams were produced without the fiber to show possible effectiveness of the fiber use. The shear performances were discussed in terms of the load—deflection response, cracking pattern and failure mode, first cracking load and ultimate shear strength. In this sense, all the non-fiber beams were failed by shear with a dramatic load drop, regardless of the tensile reinforcement amount, before the yielding of reinforcement and they produced no deflection capability. The test results showed that while the inclusion of steel fibers to the UHPC mixture with low reinforcement ratios changed the failure mode from the shear to flexure, it significantly enhanced the ultimate shear strength in the case of higher reinforcement ratio through the SF-UHPC’ superior mechanical properties and fibers’ crack-bridging ability.