The purpose of this study was to analyze the comparison of the number of Laban leaf extracts from geothermal and non-geothermal regions, calculating yield values, and identifying active compounds in Laban leaves taken from geothermal and non-geothermal regions in Aceh Besar, Aceh Province. The study was started by extracting Laban leaves by maceration method, using n-hexane, ethyl acetate and methanol for 3 x 24 hours. Then the extract was concentrated with a Rotary evaporator, calculated yield value, and phytochemical test. The results of the identification showed that there were differences in extract weight and weight of yields in the leaves in geothermal and non-geothermal regions. In n-hexane extract, the weight difference of extract was 37.6 g, and the difference in weight of the yield was 1.25%. In ethyl acetate extract the extract weight difference was 98.57 g, and the weight difference in yield was 3.28%. Methanol extract weight difference is 427.33g, and the difference in weight of the yield was 11.59%. The biggest difference is found in methanol extract.

BACKGROUND: One of the natural ingredients that can be used as a substitute for preventing dental caries is the methanol extract of Laban leaves. Laban contains various compounds that are known to have antibacterial properties. The traditional ability of Laban Leaf Methanol Extract has been proven to treat diseases caused by pathogenic microorganisms. AIM: The aim of the study was to analyzing the activity test of Laban leaf methanol extract toothpaste (Vitex pinnata) against the growth of Streptococcus mutans bacteria. METHODS: This type of laboratory experimental research (true experimental design) with post-test only control group design. This research was conducted at the Poltekkes Pharmacy Laboratory of the Ministry of Health in Aceh in July-August. The research sample consisted of Laban leaves collected randomly in the Ie Seu Um Geothermal area of Selawah Agam Aceh Besar. Data analysis was carried out descriptively. Antibacterial activity data were statistically processed with one-way ANOVA at a confidence level of α 0.05. RESULTS: The organoleptic test results observed for 3 weeks showed that there were differences in color and aroma; the higher the concentration of the resulting color, the darker it was, and resulting in a soft, sweet taste in the toothpaste of laban leaf methanol extract. The pH acidity test results showed an effect of storage time (the week I, week II, and week III) on toothpaste’s pH value. The viscosity test results showed that the toothpaste formula combined with the methanol extract of Laban leaves met the SNI 12-524-1995 standards regarding toothpaste. Toothpaste formula F3. 4.5% has the greatest viscosity, ranging from 241.0 (PDAs), and followed by F2. 240.4%, and F1. 237.6%. The higher the active ingredient concentration, the greater the viscosity value. The foam formation test results show that the foam’s stability is greatly influenced by particle size. The more the particle size, the lower the foam stability. ANOVA test results based on the concentration of significance value (0.000) <0.05, there is a difference in the number of bacterial colonies based on the concentration variable. ANOVA test results based on the repetition of significant values (0.423)> 0.05, which means that all repetitions have the same effect on the number of colonies, so there is no difference in the number of colonies based on the repetition variable. CONCLUSION: Minimum inhibitory concentration of toothpaste laban leaf methanol extract against S. mutans growth was aimed at a concentration of 4.5%, with an average number of bacterial colonies 108.5 × 10−7 CFU/mL).

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