The aim of the study was to test the effects of various concentrations of zinc sulfate (ZnSO4) on the radial growth of Trichoderma harzianum and Leucoagaricus gongylophorus in Petri dishes. In addition, we investigated the acceptance of baits containing live propagules of the encapsulated T. harzianum fungus and baits containing ZnSO4 by foraging leafcutter worker ants, A. sexdens, as well as their effects on the colonies of these ants. For the in vitro test, the design was completely randomized using ten replicates of a 2 × 7 factorial scheme: two species of fungi (T. harzianum and L. gongylophorus) and seven concentrations of ZnSO4. For bait acceptance tests, four treatments were used: baits without the mycelium and ZnSO4 (T1, control), baits containing encapsulated T. harzianum mycelium (T2), baits containing ZnSO4 (T3), and baits containing zinc sulfate and the encapsulated T. harzianum (T4). Each treatment was tested in duplicates using eight colonies. In the in vitro test, a ZnSO4 concentration of 0.25 g/L was sufficient to completely inhibit the development of L. gongylophorus without affecting the development of T. harzianum. In the bait acceptance test, the workers accepted ~55% of the bait combination of ZnSO4 and T. harzianum. This suggests the potential of treatment T4 in controlling leafcutter ants. Moreover, treatment T4 is environmentally friendly.
As formigas-cortadeiras têm grande importância econômica, já que podem cortar e utilizar ampla diversidade de espécies vegetais cultivadas pelo homem. Este trabalho estudou os efeitos do zinco nas formas de cloreto e sulfato sobre o sistema imune da formiga-cortadeira Atta sexdens. Os experimentos consistiram na imersão das formigas em soluções de sulfato de zinco (ZnSO4) e cloreto de zinco (ZnCl2). Para a contagem total de hemócitos, foi feita a retirada da hemolinfa, e a concentração de hemócitos foi submetida à análise de variância pelo teste F (p ≤ 0,05) seguida por regressão polinomial (p ≤ 0,05). A encapsulação foi medida por meio do implante de um monofilamento de nylon, um antígeno inerte, nas operárias e taxa média de encapsulação dos diferentes tratamentos foi submetida à análise da variância pelo teste F (p ≤ 0,05) seguido pelo teste Scott-Knott a 5% de probabilidade. As diferentes formas de sais de zinco diminuíram significativamente a concentração de hemócitos das operárias. A taxa de encapsulação não apresentou diferenças significativas entre os tratamentos. O sulfato de zinco apresentou-se mais eficiente na redução dos hemócitos em relação ao cloreto de zinco, pois reduziram significativamente a imunidade desses insetos.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.