We have cloned two cDNAs from the pituitary gland of blue gourami (Trichogaster trichopterus), coding for the subunits of the gonadotropin hormones GtH-I and GtH-II. The two cDNAs were sequenced and subjected to sequence analysis. We have found that the deduced amino acid sequences of both cDNAs were most similar to their striped bass counterparts. The GtH-I subunits of blue gourami and striped bass shared 73% of their residues, and the GtH-II subunits 84%.
The cloning of the cDNAs ofGtH-I and GtH-II has enabled us to measure the expression of their respective mRNAs in the pituitaries of female blue gourami at different stages of the reproductive cycle. The highest levels of GtH-I and GtH-II mRNA were found in specimens classified as high vitellogenic and in females that were at the final stages of oocyte maturation.
The elastic and inelastic scattering of n particles by ¹i and Pb has been investigated at an incident energy of 139 MeV. The elastic cross sections have been analyzed in terms of the optical model using a six-parameter Woods-Saxon potential. The data for 5 ¹i are sufficient to eliminate the discrete ambiguity in the strength of the potential; the single potential which fits the data has a well depth of 116 MeV and a volume integral J/4A of 298 MeV fm3.For Pb the discrete ambiguity could not be resolved. This outcome is consistent with recently developed criteria for experimental data necessary to resolve the discrete ambiguity. A discussion of the discrete ambiguity, in particular the A dependence, is given, and it is shown that measurements at higher energies are required to resolve the ambiguity for Pb. The inelastic cross sections for transitions to the 1.45-MeV (J~=2+), 2.46-MeV (4+), 4.47-MeV (3 ) states in 58Ni and the 2.62-MeV (3 ) state in Pb have been analyzed with distortedwave Born-approximation (DWBA) calculations using collective-model form factors. The results are consistent with previous analyses of lower-energy data.
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