The aim of the research was to study the effect of the type of higher nervous activity on the excretory function of the mammary gland. Cows are classified according to the types of higher nervous activity (HNA): Type I -strong, balanced, mobile; Type II -strong unbalanced; Type III -strong inert; Type IVweak breaking. Under standard milking conditions, in cows of the I HNA type there were observed: a tendency or a significant increase in single milk yield up to 14.4% and milking time up to 23.4% in the morning milking in comparison with other types. The conditioned stimulus "foreign" milkmaid, when applied once or twice, caused an increase in the number of milk flow cycles in I HNA type cows to 43.6% per lunchtime milking; and in type II, an increase in their number to 113% with a decrease in the duration of one cycle to 69.6% both in the morning and lunchtime milking, compared with standard conditions. The following positively correlates with the intensity of milk flow: single milk yield, the milk flow rate for the first and second minutes of milking and maximum, the percentage of milk yield for the first and second minutes, the number of milk production cycles for two, three minutes and in general for milking. Negatively correlates the time of milk yield of the first 50 and 100 g of milk, the achievement of the maximum rate of milk flow and the total milking time. The process of inhibition of milk flow weakens these connections. The type of HNA in cows affects the relationship of indicators of milk flow both under conditions of varying degrees of readiness for milk flow, and action of conditioned and unconditioned stimuli on the animals used in the experiment.