Few microbial studies in aquaponics, a growing trend in food production, have been conducted to determine food safety status. The aim of this study was to determine the food safety status and the effectiveness of ultraviolet treatment (15 W, luminous flux of 900 lm) as a food safety intervention in reducing the microbial loads of the water system in a model aquaponic unit growing lettuce, basil, and barramundi (Australian Sea Bass). Sweet basil, bibb lettuce, water samples, and fish swabs were collected throughout the 118-day production period, and microbial analysis was conducted in triplicate for the presence of E. coli O157:H7, Salmonella spp., and the prevalence of aerobic plate counts (APC), coliforms, and fecal coliforms in these systems. Absence of foodborne pathogens was confirmed using ELISA technology and enumeration through petrifilms (coliform/E. coli). A significant increase was observed in aerobic plate counts over the trial period (1 to 3 log 10 CFU·mL −1 ) in the presence and absence of UV (p > 0.05). Ultraviolet treatment did not significantly reduce the APC or coliform counts when compared to the control system samples. Future work should focus on improving the unit design, the evaluation of bio-solid filtration, and other food safety interventions.

Aquaponics is an agricultural practice incorporating aquaculture and hydroponic principles. This study assesses the current system design and production practices of the aquaponic industry, compares these metrics by stakeholder group, identifies trends, and provides recommendations for future development. An electronic survey of aquaponic stakeholders was conducted from December 2019 to June 2020 targeting hobbyists, producers, and educators from various aquaponic-focused professional associations, email and social media groups. Of 378 total responses, 84% came from the United States and were clustered in plant hardiness zones five to nine. Aquaponic systems were commonly homemade/do-it-yourself (DIY), many of which incorporated commercially available (turn-key) technology. Most growers used coupled systems that integrated recirculating aquaculture systems and either deep-water culture (DWC) or media bed hydroponic units. Common plant lighting sources were sunlight and light emitting diode (LED). Water sources were typically municipal or wells. Personal labor input was typically less than 20 hrs/wk. Funding sources were primarily personal funds, followed by government grants, and private investor funds. System sizes varied greatly, but the median area was 50 to 500 ft2 for hobbyists and educators and 500 to 3,000 ft2 for producers. Respondents commonly sold vegetable produce, training and education, food fish, and microgreens. Tilapia and ornamental fish were commonly grown, with 16 other species reported. Common crops were lettuce, leafy greens, basil, tomatoes, peppers, and herbs with many additional lesser-grown crops reported, including cannabis. Overall, the industry still growing, with a large portion of stakeholders having less than two years of experience. However, veteran growers have remained in operation, particularly in the producer and educator groups. The survey results suggest a shift away from outdoor systems, media beds, tomatoes, ornamental fish, and perch production, and a shift toward decoupled systems, DWC, drip irrigation, and wicking beds, larger system area, leafy greens, and trout/salmon production compared to previous industry surveys. The reduced diversity of plant species grown suggest some level of crop standardization. Commercial producers tended to sell more types of products than other stakeholders, suggesting that diversification of offerings may be key to profitability. The combined production area specified by respondents indicates the industry has grown substantially in recent years. Finally, the presence of bank loan-funded operations suggests increased knowledge and comfort with aquaponics among lenders.

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Raised bed production trials were conducted to evaluate the effectiveness of effluent from a biofloc-style recirculating aquaculture system producing nile tilapia (Oreochromis niloticus) as nutrient-rich irrigation water for fall ‘Celebrity’ tomato (Solanum lycopersicum) production. The objective of this study was to provide baseline vegetable production data and justification for using aquaculture effluent as a water and nutrient resource. The experiment was a split-plot, randomized block design with three treatments: aquaculture effluent, granular fertilizer, and fertigation. Tomato seeds were sown in June, transplanted in August, and grown until Oct. 2019 in nine replicated raised beds. Conventional field tomato production practices were followed throughout the trial, and data were collected for tomato fruit yield, market quality, size, leaf greenness (SPAD), and foliar nutrient analysis. Fruit yield was similar between fertigated and aquaculture effluent treatments, with granular fertilizer resulting in yield that was significantly lower (P ≤ 0.033). SPAD measurements were similar among treatments. All nutrients met or exceeded sufficiency ranges. Foliar nutrient analysis revealed no significant difference for nitrogen, phosphorus, potassium, magnesium, calcium, boron, zinc, manganese, and iron among treatments. Sulfur and copper levels were significantly lower (P < 0.05) with aquaculture effluent treatment as compared with the granular and fertigated treatments. Overall, tomato production using aquaculture effluent as a water and nutrient supplement produced similar yields to commercial practices, making it potentially viable for producers.

Introduction The design and development of steam-injection fields is a mature subject. Because the tubulars in these wells invariably experience inelastic loading, issues such as the effects of temperature on the static and cyclic (fatigue) material properties become important. The original papers that address steam-injection casing-design issues date back to the 1960s. These studies include temperature prediction (Leutwyler and Bigelow 1965), casing-stress analysis (Willhite and Dietrich 1967), and development of design guidelines (Holliday 1969). Though these works acknowledge the role of temperature on the static and cyclic material properties, data on the cyclic thermal properties of oil-country tubular-goods steels rarely appear in oilfield literature (Placido et al. 1997; Maruyama et al. 1990). This paper presents a mathematical model of casing strings subjected to thermal loads in steam-injection wells. The model includes the effects of temperature on material properties and the effects of wellbore curvature and prestress during the heating cycle. Several counterintuitive aspects of the casing-stress state during cooling/unloading are examined. Further, the general equations are shown, with appropriate simplifications, to reproduce the earlier work (Willhite and Dietrich 1967; Holliday 1969). Example calculations are used throughout to illustrate key insights.