In view of its potential for biological tissues analyses at a molecular level, Raman spectroscopy in optical range has been the object of biomedical research for the last years. The main aim of this work is the development of Raman spectroscopy for organic content identifying and determination of biomarkers of saliva at a molecular level for periodontitis diagnostics. Four spectral regions were determined: 1155 and 1525 cm −1 , 1033 and 1611 cm −1 , which can be used as biomarkers of this widespread disease.An informative volume deposition on the dried saliva drop (50× magnifications)

In view of its potential for caries diagnostics, fluorescence spectroscopy in optical range has been the object of research for the last years. In the frames of this work the comparative investigations in this direction were accomplished with the help of fibre optics spectrometer and lasers at the wavelengths of 473 nm, 532 nm, and 633 nm. Together with spectroscopic investigations, the element analysis of enamel (of sound teeth and teeth with caries lesions) was fulfilled with the help of laser mass-spectrometer.

Abstract:In view of its potential for dental diagnostics, noninvasive optical methods have been the object of research for the last years. Different techniques (spectroscopy, microscopy, and tomography) are used to discriminate between caries and sound enamel. The main aim of this paper is to determine a simple way to see incipient caries with the naked eye. Relative parties R of fluorescence intensities from the sound enamel (squares) and white spots (rings) as a function of excitation wavelength λexc

In view of its wealth of molecular information, Raman spectroscopy has been the subject of active biomedical research. The aim of this work is Raman spectroscopy (RS) application for the determination of molecular biomarkers in saliva with the objective of early periodontitis detection. As was shown in our previous study, carotenoids contained in saliva can be molecular fingerprint information for the periodontitis level. It is shown here that the carotenoid RS lines at wavenumbers of 1156 and 1524 cm −1 can be easily detected and serve as reliable biomarkers of periodontitis using resonance Raman spectroscopy of dry saliva.

The aim of the present study was to investigate the fluorescence properties of dental calculus in comparison with the properties of adjacent unaffected tooth structure using both lasers and LEDs in the UV-visible range for fluorescence excitation. The influence of calculus color on the informative signal is demonstrated. The optimal spectral bands of excitation and registration of the fluorescence are determined.Excitation wavelength λ, nm Fuorescence intensity, a.u. The fluorescence spectra of calculus and hard tissues of teeth excited with the help of LEDs at the wavelengths of 632 nm for dark (1) and light (2) calculus, dentin (3) and enamel (4)