The purpose. Perfecting procedure of determination of the content of carbohydrate in nodes of tillering of plants of winter wheat by anthrone method in view of biology of the crop, features of their selection, storage and fixation. Methods. The content of carbohydrate was determined with anthrone method. Assessment of cultivars was carried out in field conditions in 2013 and 2014 by biochemical and agrobiological methods. Results. Dynamics is studied of accumulation of carbohydrate, formation of frost resistance and growth of plants of winter wheat depending on favorable weather conditions for training. Efficiency is proved of the offered developments of anthrone method for determination of the content of carbohydrate in nodes of tillering of plants of winter wheat. Conclusions. Use of the improved technique for determination of the content of carbohydrate with simultaneous fixation of critical temperature of frost killing and phase of growth allows assessing general state of plants of winter wheat and predicting perspectives of their further wintering in view of long-term forecasts of weather environment.