Aim of the Study: The phytoconstituent 6-heptadecylcyclohex 3-ene-1 carboxylic acid isolated from the methanol extract of Dichrotachys cinerea Wight. stem bark was evaluated for hepatoprotective activity against CCl4 induced toxicity. Materials and Methods: The constituent 6-heptadecylcyclohex 3-ene-1 carboxylic acid isolated from the methanolic extract of D. cinerea and the structure was confirmed by spectroscopic studies. Hepatoprotective property was screened in male wistar strain rats. The parameters studied were estimation of liver function serum markers such as serum total bilirubin, total protein, alanine transaminase, aspartate transaminase, alkaline phosphatase  and histological profile of the liver tissue. Results: The LD50 of methanolic extract and constituent, 6-Heptadecylcyclohex -3-ene-1 carboxylic acid were evaluated and found to be 500 and 100 mg/kg body weight respectively. The hepatoprotective activity of constituent was more significant as similar to the standard hepatoprotective drug silymarin. The histological profile of the liver tissue showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration as similar to the controls. Conclusion: The methanolic extract of D. cinerea stem bark and the phytoconstituent 6-heptadecylcyclohex-3-ene-1 carboxylic acid showed significant protection from CCl4 induced liver damage.

To evaluate phytochemical, heavy metal, pesticide, microbial assay, and also FTIR studies of Angiatico-2 (A-2) an Electrohomeopathic remedy to evaluate safety and efficacy for its usage.     Electrohomeopathy / Electropathy is one purely herbal medical system invented by Italian C. C. Mattie (1809-1896) has been practiced since the 1860s across the world. Electrohomeopathy has its own unique principles, plants selection, the process of remedies preparation, diagnosis, selection, and combination of drugs for different diseases, dosage, and treatment methods. In India, it is estimated that there are about 450-500 institutions imparting education and research, about 4.5 to 5 lacks practitioners are practicing and millions of population getting benefits currently. The government of India initiated steps to recognize Electrohomeopathy medical system under the constitution. Unfortunately, there is very limited scientific evidence to evaluate the safety, efficacy, phytochemical, and pharmacology studies in Electrohomeopathy. We prepared Angiatico-2 an Electrohomeopathic remedy as Mother Solution and D4 dilutions and evaluated it’s phytochemical screening, TLC, FTIR spectrum, Physical and Chemical nature, presence or absence of heavy metals, Pesticide, Food Additives and Microbial   under NABL standards for its safety usage. Electrohomeopathic herbal remedy Angiatico-2 prepared by using 7 non poisonous plants by Krauss method under standard condition and not shown any heavy metals, pesticide presence and also no microbial presence above the limit level of FSSAI standard. Present studies of Electrohomeopathic herbal remedy Angiatico-2 preparation and its screening in standard scientific laboratory condition,   TLC & FTIR studies may become reference standards for the manufacture of Angiatico-2 for bulk quantity at industrial level for. The present research investigations outcome with reproducibility may become standard markers or signatures to assess the quality and safe use of Angiatico-2.