This study describes a simple one-pot synthesis of N-pyridin-3-yl-benzenesulfonamide and its antimicrobial activity. The reaction of benzene sulfonyl chloride with 3-aminopyridine in the presence of aqueous Na2CO3 and HCl as a scavenger furnished the sulfonamide in 93.3% yield. The structure of the synthesized compound was established using the spectral data obtained from FTIR, 1HNMR and 13CNMR). All spectrometric spectrum bands and peaks obtained were sulfonamide-related. The antibacterial test carried out against Gram-positive bacterium (Staphylococcus aureus) and Gram-negative bacteria (Salmonella typhi and Eschericha coli) tested upon at concentrations 150, 100, 50 and 25 mg/ml revealed that the synthesized N-pyridin-3yl-benzenesulfonamide possesses great antimicrobial activity.