Introduction:The anterior cruciate ligament (ACL) reconstruction with pes anserinus tendons has been increasingly used throughout the last years. Although less invasive compared to other autologous grafts, a reduction of internal rotation and flexion strength after gracilis and semitendinosus harvesting has been reported. Harvesting one tendon instead of two from the pes anserinus can reduce the deficit of the knee flexor strength and improve the functional recover without weakening the reconstructed ligament. Methods: Forty-five (45) patients who had ACL reconstruction with triple semitendinosus graft (ST3) have been compared with other 45 similar patients who had ACL reconstruction with double gracilis-semitendinosus tendons (GST). Patients have been evaluated at a minimum of 12 months after surgery: IKDC scale, KT-1000, One Leg Hop Test for the objective stability; Isokinetic test for the strength; Tegner scale, Lysholm and IKDC subjective evaluation form for the function. Results: No differences have been detected between the groups for the objective item assessed.Male patients' subjective IKDC score was statistically better for the ST3 group. Recreational soccer players showed a higher Lysholm and subjective IKDC score in ST3 group compared to GST group. There was no difference regarding the return to sport. Conclusion: ST3 guarantees the same objective knee stability compared to a GST. It is a viable option for ACL reconstruction that allows a better preservation of patient's anatomy and a less invasive harvesting surgery. Level of evidence: III b, case control study.