Identification of Lactic Acid Bacteria (LAB) has been carried out based on the catalase test and Gram staining of Etawa pure Goat Milk samples that obtained from Kopelma Village, Darussalam District, Banda Aceh City, Aceh Province. De Man, Rogosa, Sharpe Agar (MRSA) media was used for LAB isolation. There were 10 isolates of goat milk bacteria that used for microscopic characteristics, morphological observations, catalase test and Gram staining. Based on the microscopic test, it was explained that all colonies had similar characteristics, that having a round shape, convex surface shape, 1-2 mm diameter and cream color. In the catalase test, the results were negative, because all isolates did not produce gas bubbles after dripped with H2O2 solution. The morphological results showed that all isolates were Gram positive, marked by purple cells and bacilli cells. Based on the Gram staining and the shape of the bacillus cells, it were suspected that milk isolates are candidates for the genus Lactobacillus. Microscopic diagnosis is only a provisional presumption. Therefore, it needs a conclusive diagnosis, such as biochemical properties which are important factors that must be carried out for the next stage.

ABSTRAKPenelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P<0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli. ____________________________________________________________________________________________________________________ Kata kunci: Ascaridia galli, ekskretori/sekretori, antibodi, ELISA ABSTRACT

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost’s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein  ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100  ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 5–10  L3 dikultur secara in vitro  dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 µg ml-1 streptomisin, 5 µg ml-1 gentamisin dan 0,25 µg ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian  menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli,  embrionated eggs