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AbstractIn this study, we analyzed the obstetric factors affecting total nucleated cells (TNC) content of cord blood units to establish the criteria for umbilical cord blood (UCB) donor selection in our geographic area.UCB was collected from normal uncomplicated pregnancies. In every case, following data were recorded: (1) gestation length; (2) type of delivery (cesarean or vaginal); and (3) newborn characteristics: weight and sex. For each sample, TNC content, percentage and number of CD34+ cells, and viability were analyzed.The results showed that TNC content increases with cord blood volume, gestational length and newborn weight. The mean blood volume and the mean TNC per unit were 42.37 ± 13.5 ml and 55.49 ± 19.4 × 107, respectively. Stepwise regression analysis revealed a positive and significant correlation (r= 0.89) between these two variables. Meanwhile the CD34+ cell content remains unchanged in deliveries at 32–40 weeks of gestation. The mean CD34+ percentage obtained was 0.37 ± 0.06, and the total number of CD34+ cells was 4.827 ± 0.8204 × 104 / mL UCB.Concluding, the maternal and obstetric factors have a significant impact on UCB cell quantity and quality. The main criteria for UCB collection and storage resulted to be: a gestational age higher than 36–40 weeks and newborn weight > 3200g; gestation number ≤ 2 and placental weight > 700g can be added to the standard criteria to improve the bank efficiency. Our results have also become helpful in evaluating stored UCB units to establish the adequacy for clinical transplant utilization.

Background and aimsLow birth weight infants are at high risk of perinatal complications and death to normal or large infants. Umbilical cord abnormalities are associated with this group of infants due to chronic aggravation of umbilical blood flow. We want to establish the anatomical features of umbilical cord who can predict the outcome of newborn.Materials and methodsAnalytical study of low birth weight newborns, with a duration of 3 years conducted in National Institute of Mother and Child Health ‘Alessandrescu-Rusescu’, Neonatology Clinic. Were monitored type of conception and delivery, umbilical cord anatomic features, Apgar score, need for invasive resuscitation at birth (oxygen – positive pressure ventilation, achieving PEEP with T piece resuscitator, intubation), outcome of newborns.ResultsLow birth weight infants studied came from 90% investigated preganat women, multigestation 62,5% and primiparous 67,5%; naturally conceived in 78,3% cases and 21,6% in vitro fertilisation; equal proportion by gender; 43% term newborn, 8,3% between 33–36 weeks of gestation, 39,1% between 28–32 weeks of gestation and 9,1% under 27 weeks of gestational age; number of days oh hospitalisation were above normal in most cases. Cord appearance in this group was normal in 79,1% of cases, lin 9,1%, hypertrophic 10,8%, excess Wharton jelly in 5,8% of cases, meconium stained 3,8%. In the series with meconium stained umbilical cord, maternal hystory was infectious type in 75% of cases, 50% under 27 weeks gestational age, 75% with Apgar score under 1 at 1 minut after birth, and all newborns required resuscitation at birth; those with excess Wharton’s jelly came in 86% of cases from primiparous, 42,8% concevied by in vitro fertilisation and multipe fetuses, all at 28–32 weeks gestational age, need for resuscitation at birth 43%; hypertrophic cord was associated with maternal pathology like placenta praevia 25% and pregnancy hypertenison 25%, all naturally concieved, 50% with gestational weeks between 28 and 32, hospitalisation over one month; lin umbilical cord was associated with 72,7% multigestation of wich 37,5% primiparous, without significant pathology, naturally concevied, 63,6% between 33 and 36 weeks gestational age, 45,1% with Apgar score 5–7 and need for resuscitation at birth.ConclusionsIn this situation, we have the confirmation that the features of umbilical cord can be the first clinical exam of low birth weight newborns who could oriented the action of neonatal team and treatment for infants.

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Uterine fibroids are the most common benign tumor that can be found in a woman's reproductivesystem. The uterine fibroid affects millions of women globally each year and we can consider thispathology, without exaggerating, an important public health problem. Our study was carried outprospectively, in 5 years (2015-2019) and enrolled 480 patients selected with uterine fibroid, from anumber of 28809 women who were hospitalized during this period in the university clinic ofObstetrics and Gynecology within the „Saint Pantelimon” Emergency Hospital, Bucharest, Romania.Patients were divided into two groups of study: Group A, those with well-defined, single or multipleuterine fibroid and Group B – patients with diffuse uterine fibromatosis. The clinical,histopathological and immunohistochemical study analyzed and monitored over time, by comparisonbetween the two groups, 52 parameters for each patient, starting with the usual epidemiologicalfactors (age, weight, height, body mass index, personal or family medical history, etc.), continuingwith those related to fertility and other clinical issues (chronic pain, metrorrhagia, number of births,recurrent miscarriage, etc.), up to intraoperative parameters (duration of surgery, blood loss, pelvicdrainage, the need for blood transfusion) and finally, histopathological aspects. We present in thisarticle some of the results of our study, those related to the quality of the endometrium in thefibromatous uterus and the influence that uterine fibroid had on the fertility of our patients.