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A study was carried out from February 2013 to January 2015 in 1650, buffaloes of organized and unorganized farms of Durg, Rajnandgaon, Raipur and Bilaspur districts of Chhattisgrah State in India, to determine the prevalence of subclinical mastitis. Milk samples were collected to detect subclinical mastitis in individual animal based on milk somatic cell count (SCC) and MCMT. The overall prevalence of buffalo subclinical mastitis accounted for 41.51% (685/1650). Prevalence of SCC was evaluated on the basis of geographical distribution, parity, age and stage of lactation, pattern of milking etc. More cases of SCC were observed in unorganized farms with Prevalence ranges from38.41% to 44.93% in Durg and Rajnandgaon, respectively. Buffaloes of 9-11 year suffered more that is 57.51% as compared to younger with early lactation (52.78%). More number of cases was observed in rainy season and least in dry climate. Prevalence % of MCMT score + was observed more with SCC range from 2.12-2.85×10 5 cells/ml of milk.

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