Chitosan is very useful in everyday life in adsorption, cosmetics, pharmaceuticals, flocculants, anticancer and antimicrobial.In this study, chitosan was synthesized from chitin extracted from crayfish. The methods such as deproteinization, demineralization, and deacetylation respectively were used in the synthesis of chitosan from crayfish. Antimicrobial activity was studied and it was found that chitosan and Moringaleaf powder were good in inhibiting the growth of microorganisms; confirmed by the results obtained from the experiments. In evaluating the antimicrobial activity, the serial dilution method was used towards Escherichia coli, Staphylococcusaureus, Salmonella typhi, Proteus bulgaris and Streptococcus pneumonia. The antibacterial activity of chitosan composite with the leaf powder of Moringa oleifera Lam., was determined, using well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration determination method. The composites show the synergistic effect at the higher chitosan to lower Moringa concentration and antagonistic effect at higher Moringa to lower chitosan concentrations in all the test organisms. The consequences of this research suggest that the chitosan, Moringaleaf powder, and their composites can be used to discover an antibacterial agent for developing new pharmaceuticals to control studied human pathogenic bacteria responsible for severe illness.Moringaoleiferais widely used in food and folk medicine; while chitosan is widely useful in food, detergents, textiles, leather, paper, pharmaceuticals, and cosmetics industries. Synergism/antagonism of Moringa-chitosan composites was based on concentrations on the tested organisms.
This study presents the mineral composition of the parts of Moringa oleifera in Zaria. Samples of the parts were wet digested and analyzed for various minerals using Varian Instrument AA240FS for the atomic absorption spectrophotometry (AAS). The contents of Zn, Fe, Ca, Cu and Mg in the parts (roots, stem barks, pods, seed cases/shell, seeds and leaves) of Moringa oleifera were found to be 0.096, 0.872, 12.317, 0.008 and 2.076 mg/g in the roots respectively; 0.022, 0.412, 0.29.397, 0.005, and 3.729 mg/g in the stem barks respectively; 0.021, 0.017, 8.048, 0.003 and 1.827 mg/g in the pods respectively; 0.019, 0.056, 6.467, 0.002, and 1.551 mg/g in the seed cases/shells respectively; 0.017, 0.029, 4.217, 0.001 and 1.616 mg/g in the seeds respectively and in the leaves 0.017, 0.205, 19.974, 0.005 and 2.494 mg/g respectively. The contents of different minerals in the parts of M. oleifera varied from parts to parts. This variation in the contents of various minerals within the parts might be attributed to the variable uptake of minerals by the plant material and variable agro-climatic conditions of the subject region. The results of the present analysis revealed that parts of M. oleifera contained a considerably high amount of Zn, Fe, Ca, Cu and Mg that can be used as a viable supplement of medicinal and dietary minerals which makes it a promising pro-medicine.
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