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Signal processing methods and constraints for discerning the fluorescence signals of the QD‐barcodes are explored. QD‐barcodes and their corresponding fluorescence spectra (see figure) require signal processing algorithms in order to be uniquely identified. Using these algorithms, we determined the number of available barcodes for use in biological detection. We also studied the impact of chemical constraints such as buffer and pH level on the barcode and read‐out design.

The electrophoretic motion of two spherical particles in an aqueous electrolyte solution in a small rectangular microchannel was studied in this paper. A theoretical model was developed to describe the electric field, the flow field, and the particle motion. A direct numerical simulation method using the finite element method is employed to solve the model. The simulation results clearly show how the presence of one particle influences the electric field, the flow field, and the motion of the adjacent particle. Such an influence weakens with the separation distance. In addition to the zeta potentials, the particles' motion depends on their sizes: the smaller particle moves slightly faster. For a faster particle moving from behind of a slower particle, the simulation results show that the faster particle will climb and then pass the slower particle when the two particles' centers are not located on the same line parallel to the applied electric field.

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