Aim: This study’s objective is to determine how polypropylene fibre impacts self-healing concrete’s flexural strength. Materials and Methods: Investigation was divided into two groups, with each group preparing 18 samples with Gpower (80 percent). Group-1 denotes conventional self-healing bacterial concrete, while Group-2 denotes polypropylene fibre reinforced self-healing bacterial concrete. The number of samples was determined using clinical software and values taken from previous research publications. The independent sample T-test, performed using SPSS software version 21, demonstrates a substantial increase in the flexural strength of the fibre reinforced self-healing concrete. Result: In comparison to conventional self-healing bacterial concrete, polypropylene fibre reinforced self-healing bacterial concrete has a 19% higher flexural strength. The significance of the events was 0.354 (p>0.05), indicating that there was no significant statistical difference. The flexural strength of polypropylene reinforced bacterial concrete had a standard deviation of 0.79623. Conclusion: According to the findings of this experiment, M20 grade self-healing bacterial concrete reinforced with polypropylene fibre has a stronger flexural strength than regular self-healing bacterial concrete.